反相高效液相色谱法测定阿霉素聚乳酸微球的含量  

HPLC determination of loading amount of adriamycin polylactic acid microspheres

作　　者：阎志飞[1] 赵志娟[1] 丁红[1] 罗斌华[1,2] 

机构地区：[1]山西医科大学药学院药剂学教研室,太原030001 [2]湖北咸宁学院药学院

出　　处：《中国药物与临床》2010年第7期754-755,共2页Chinese Remedies & Clinics

基　　金：山西医科大学青年基金(02200809)

摘　　要：目的采用反相高效液相色谱法测定阿霉素聚乳酸微球中的含量。方法采用AgilentEclipseXDB-C18色谱柱(150mm×4.6mm,5μm),以乙腈-甲醇-水(4∶5∶1)为流动相,检测波长为233nm。结果阿霉素在5～50μg/ml与峰面积呈良好的线性关系(r=0.9994),方法平均回收率为99.92%,RSD=0.82%。结论反相高效液相色谱法测定阿霉素聚乳酸微球的含量简便易行,专属性强,灵敏度高,重复性好,结果准确。Objective To determine the loading amount of doxorubicin polylactic acid microspheres by RP- HPLC. Methods An RP-HPLC method was employed for determination using Agilent Eclipse XBD-C18 column （150 mm×4.6 mm, 5 μm） with a mobile phase consisted of acetonitrile-methanol-water （4∶5∶1）. The length of wavelength was 233 nm. Results Good linearity was achieved in the range of 5～50 μg/ml （r=0.9994）. The recovery was 99.92% with RSD of 0.82%. Conclusion This method appeared easy-to-use, specific and sensitive, reproducible and accurate.

关 键 词：色谱 高效液相法 阿霉素 微球体 聚乳酸 

分 类 号：R944[医药卫生—药剂学]