人肠道乳酸杆菌的荧光定量PCR定量检测方法的研究  

Studying of Detecting the Lactobacillus Quantitatively by Fluorescent Quantificative PCR

作　　者：张立营[1] 侯方高[1] 冯玉奎[1] 孙宇[2] 孙英姿[1] 高波[1] 

机构地区：[1]济南军区青岛第二疗养院检验科,山东青岛266071 [2]北京305医院检验科,北京100050

出　　处：《中国误诊学杂志》2010年第17期4033-4035,共3页Chinese Journal of Misdiagnostics

基　　金：青岛市公共领域科技支撑计划项目(编号:09-1-1-28-nsh)

摘　　要：目的应用实时荧光定量PCR技术对健康人粪便内乳酸杆菌进行定量分析,建立乳酸杆菌的实时荧光定量PCR检测方法。方法依据乳酸杆菌16S rDNA序列设计属特异性引物,应用荧光定量PCR技术检测乳酸杆菌的16SrDNA,对粪便中的乳酸杆菌进行定量检测和分析,并和用传统方法所获得的结果进行比较。结果结果显示荧光定量PCR检测乳酸杆菌和传统方法检测乳酸杆菌之间无统计学差异(P>0.05)。结论荧光定量PCR技术比传统方法特异性高、敏感性强,临床上可用此方法对患者肠道乳酸杆菌进行定量分析。Objective To quantitatively detect the Lactobacillus in the fecal samples from healthy population by Fluorescent quantificative PCR（FQ-PCR）.Methods Genus-specific primer of Lactobacillus was designed for 16S rDNA-targeted PCR.Bacterial genome DNA in fecal samples was extracted from the heathy population and detected by FQ-PCR,respectively.And were compared with the result by the conventional method.Results The range of bacteria by FQ-PCR was nearly equal to the result by the cultivation techniques.Conclusion FQ-PCR is a method with high specificity and sensitivity,and it can be applied to the quantification of bacteria in the intestine and saves more time and labor.

关 键 词：乳杆菌属/遗传学 聚合酶链反应 

分 类 号：R378.2[医药卫生—病原生物学]