乳酸氧化酶在复合功能铝膜中的固载研究  

Immobilization of Lactate Oxidase Within a Hybrid Mesoporous Membrane

作　　者：付文升 罗兴萍[1] 黄俊菲[1] 曹梨梨[1] 黄小龙[1] 谭裕玲[1] 贺娇[1] 

机构地区：[1]重庆师范大学化学学院,重庆400047

出　　处：《重庆师范大学学报（自然科学版）》2010年第4期61-64,共4页Journal of Chongqing Normal University:Natural Science

基　　金：国家自然科学基金(No.20801062);重庆市自然科学基金(No.CSTC2009BB4055)

摘　　要：在有序多孔的阳极氧化铝膜腔体内,以F127为表面活性剂,采用压力诱导的方法制备出孔径约为12nm的介孔二氧化硅一维纳米材料。制得的复合功能性铝膜,先用烧结的方法除去表面活性剂F127,然后将3-胺基丙基-三甲氧基硅(APTMS)固定到介孔的表面上,通过戊二醛(GA)的交联作用使乳酸氧化酶(LOD)固载,制备出具有生物催化活性的复合功能性铝膜。实验结果表明,复合功能性铝膜催化乳酸溶液时,当其流速很慢(0.3mL/min)且浓度较低(5μM)时可以获得83.8%左右的较高的转化率。The hybrid mesoporous membrane was prepared by using Pluronic F127 （（PEO） 106 （PPO）70 （PEO）106 ） as a structure-directing agent. Columnar mesoporous silica was formed inside the anodic aluminium membrane （AAM） pores and had a length of ca. （ 50 ±2） μm. Nano-fiber with the diameters of the pores being about 12 nm was aligned along the long axis of the AAM pore walls. The lactate oxidase （LOD） encapsulation was done by covalent attachment on the inner wall of the silica mesopores. After removing of Pluronic F127 inside the silica mesopores by calcination, the 3-aminopropylethoxysilane （APTMS） was immobilized on the inner pore surface. Then, the LOD was covalently attached via a linker of glutaraldehyde （GA） and the LOD-M was obtained. The resulting LOD-M was applied for conversion of lactic acid to pyruvic acid. The LOD-M can be used for conversion of lactic acid by using a conventional ？ ltration apparatus. The proposed scheme allows conversion of lactic acid without separation of the mesoporous silica host from the reaction media. We compared the morphology of the mesoporous material after imobilization of enzyme by TEM and isotherm. The effect on enzyme activity, such as concentration of L-lactate, flow speeds and storage time, was also tested.

关 键 词：阳极氧化铝膜 乳酸氧化酶 介孔二氧化硅 合成 

分 类 号：O611.6[理学—无机化学]