裸质粒转染细胞的可行性研究  

Feasibile Study of Naked Plasmid Transfection Cell

作　　者：胡伊乐[1] 曹靖[2] 臧卫东[2] 徐玉英[2] 涂心明[1] 

机构地区：[1]河南科技大学医学院,河南洛阳471000 [2]郑州大学基础医学院,河南郑州450052

出　　处：《河南科技大学学报（医学版）》2010年第2期83-85,共3页Journal of Henan University of Science & Technology:Medical Science

基　　金：河南科技大学科研基金(0827053)

摘　　要：目的验证裸质粒转染细胞的可行性与合适的转染的条件。方法取PCDNA3.1(+)-EGFP与pEGFP-C3两种绿色荧光载体,以不同的浓度转染细胞,观察转染效果,选取转染效果较好的质粒浓度进行动物实验,进一步验证其效果。结果真核载体可直接通过细胞膜进入细胞并表达绿色荧光。结论真核载体可直接通过细胞膜进入细胞,但其通过效率与自身的结构与质粒浓度有一定关系。Objective To test the feasible study of naked plasmid transfection cell and suitable transfecting condition. Methods Diverse concentration plasmids are transfected to NIH3T3-cells and then selecting suitable concentration plasmid to inject cavitas subarachnoidealis of rats.Detect their biologic effect and expressive locations by fluorescent microscope. Results Initial eukaryotic vector can transfect cell envelopment and express green fluorescent protein. Conclusion Eukaryotic vector can transfect cell envelope directly,but its efficiency is related to plasmid＇s construction and concentration.

关 键 词：绿色荧光蛋白 真核载体 基因表达 

分 类 号：Q786[生物学—分子生物学]