九香止泻片对腹腔注射细菌致死量小鼠白细胞、淋巴细胞计数的影响  

Effects of Jiuxiang Zhixie tablets on IL-8,leukocyte count and lymphocyte count in mice with intraperitoneal injection of lethal dose bacteria

作　　者：彭芝配[1] 张金慧 李为[3] 滕久祥[1] 伍参荣[1] 崔娜妮[1] 郑世昌[1] 

机构地区：[1]湖南中医药大学,湖南长沙410007 [2]韶关医学院,广东韶关512000 [3]湖南中医药大学第一附属医院,湖南长沙410007

出　　处：《湖南中医药大学学报》2010年第5期16-18,共3页Journal of Hunan University of Chinese Medicine

基　　金：湖南省教育厅重点项目(05A032)

摘　　要：目的观察九香止泻片(又名秦香止泻肠溶片)对腹腔注射致死量大肠杆菌、痢疾杆菌小鼠白细胞(WBC)、淋巴细胞(LYM)计数的影响,探讨其治疗湿热泄泻的作用机制。方法分别给小鼠腹腔注射致死量大肠杆菌、痢疾杆菌等不同细菌菌悬液制作腹泻感染动物模型,除模型组在动物临死之前采用无菌操作心脏采血外,其余各组在处死动物之前采用无菌操作心脏采血,然后采用瑞氏染色法对小鼠的WBC、LYM进行计数。结果与模型组比较,九香止泻片各剂量组具有明显降低感染模型小鼠WBC计数及增加LYM的作用,差异具有统计学意义(P﹤0.05),且九香止泻片高剂量组与肠康片组比较,差异具有统计学意义(P﹤0.05)。结论提示九香止泻片具有调节WBC及LYM的作用,可能是其治疗湿热泄泻的作用机制之一。Objective To observe the effects of Jiuxiang Zhixie tablets（or Qinxiang Zhixie tablets） on interleukin-8（IL-8） and leukocyte（WBC） and lymphocyte（LYM） count in mice in-traperitoneal injection of lethal dose of escherichia coli and shigella dysenteriae,and explore its mechanism for damp-heat diarrhea.Methods The mice model were induced by intraperi-toneal injection of lethal dose of E.coli and shigella dysenteriae and so on.Except for that the blood was collected from the heart before killing the animals,all the blood were got after killing animals,and then,IL-8 contents was measured with double antibody sandwich enzyme linked immunosorbent assay（ELISA）,and WBC and LYM with Wright staining.Results Com-pared with model group,Jiuxiang Zhixie tablets significantly reduced inflammatory cytokines IL-8 concentration and WBC count,increased LYM counts in infected mice（all P0.05）.In addition,the difference between high dose Jiuxiang Zhixie tablets and Changkang tablet group was significant（P0.05）.Conclusion Jiuxiang Zhixie tablets may be involved in the regulation of inflammatory mediators,inhibit leukocyte chemotaxis at molecular level,which may be one of the mechanisms for acute infectious diarrhea.

关 键 词：九香止泻片 白细胞 淋巴细胞 作用机制 小鼠 

分 类 号：R285.5[医药卫生—中药学]