电针结合经颅磁刺激对局灶性脑缺血大鼠NeuN表达的影响  

Effects of electro-acupuncture combined with rTMS on expression of inherent neural stem cells in local cerebral ischemia in rats

作　　者：黄国付[1] 黄晓琳[2] 

机构地区：[1]武汉市中西医结合医院针灸科,湖北武汉430022 [2]华中科技大学同济医学院附属同济医院康复医学科,湖北武汉430030

出　　处：《湖南中医药大学学报》2010年第5期66-69,共4页Journal of Hunan University of Chinese Medicine

基　　金：国家自然科学基金资助课题(30672216);武汉市卫生科研基金项目(WX08A01;WZ08B02)

摘　　要：目的探讨电针(EA)结合重复经颅磁刺激(rTMS)对局灶性脑缺血大鼠神经核抗原(NeuN)表达的影响及其治疗缺血性脑损伤的作用机制。方法取Wistar大鼠120只,采用线栓法制备大鼠大脑中动脉闭塞模型,随机分为正常组、模型组、电针组、rTMS组和电针结合rTMS组(结合组),通过免疫组化检测脑缺血后7、14 d与28 d 3个不同时相、不同脑区NeuN与NeuN/BrdU表达的变化,并观测其神经功能评分。结果海马颗粒下区(SGZ)和侧脑室下区(SVG)缺血后7 d,NeuN和NeuN/BrdU阳性细胞数量的表达明显增加,差异有统计学意义(P<0.05),14 d表达达到高峰,28 d表达开始下降,其中增加最明显的为结合组,电针组和rTMS组也有明显增加,但电针组和rTMS组之间无显著性差异,模型组增加最少;从组间比较看,电针组、rTMS组、结合组与模型组在7、14 d时差异有统计学意义(P<0.05),结合组在7、14 d时均高于电针组和rTMS组,差异有统计学意义(P<0.05)。电针组、rTMS组和结合组各时相神经功能评分均较模型组改善,差异有统计学意义(P<0.05),尤以结合组为明显。结论电针结合rTMS对脑卒中后神经功能的恢复具有显著的促进作用,促进缺血侧不同脑区NeuN的表达可能是其治疗缺血性脑卒中的重要作用机制之一。Objective To investigate the effects of electro-acupuncture（EA） combined with repetitive transcranial magnetic stimulation（rTMS） on expression of NeuN after focal cerebral is-chemia in adult rats and the mechanism of EA combined with rTMS in treating ischemic brain injury.Methods The models were made with the middle cerebral artery occlusion.All the 120 one Wistar rats were randomly divided into normal group,model group,EA group,rTMS group and EA combined with rTMS group.The expressions of neuronal nuclear antigen（NeuN） and NeuN/BrdU in different encephalic regions of ipsilateral hemispheres were detected by immunohisto-chemical staining and the neurologic impairment rating was observed at the 7th,14th and 28th days after infarction respectively.Results The number of NeuN increased at day 7 after MCAO（P0.05）,reached maximum at day 14（P0.05）,and decreased markedly at day 28,but it was still elevated compared with that in normal group.There were significant increase in EA group,rTMS group and EA＋rTMS group,but no more so much increase in model group,and not more differences between EA and rTMS groups;there were significant differences between EA,rTMS and EA =rTMS groups with model group at day 7 and 14（P0.05）.There were significant differences between EA ＋rTMS group and EA and rTMS groups（P 0.05）.However,there were no statistic differences compared in EA,rTMS and EA＋rTMS groups and model group at day 28（P0.05）.The improvement of neural motor function was much better in EA,rTMS and EA＋rTMS groups compared with model group（P0.01）,and it was the most obvious in EA＋rTMS group.Conclusion EA combined with rTMS can promote the functional recovery after cerebral arterial thrombosis,promote the expression of NeuN in different encephalic regions of ipsilateral hemispheres,which may be one of the important mechanisms of EA combined with rTMS in treating ischemia brain injury.

关 键 词：脑缺血 电针 重复经颅磁刺激 神经核抗原 大鼠 

分 类 号：R245[医药卫生—针灸推拿学]