咪唑键合硅胶液相色谱固定相的正相色谱行为  

Normal Phase Interaction of Imidazole Functionalized Silica Stationary Phase for Liquid Chromatography

作　　者：李广[1,2] 刘霞[1] 蒋生祥[1] 

机构地区：[1]中国科学院兰州化学物理研究所,甘肃兰州730000 [2]甘肃省分析测试中心,甘肃兰州730000

出　　处：《分析测试学报》2010年第6期621-624,共4页Journal of Instrumental Analysis

基　　金：国家自然科学基金资助项目(20775081;20875096);国家科技重大专项资助项目(2008zx05011)

摘　　要：该文将咪唑键合硅胶液相色谱固定相填充到毛细管中,在自制的微柱液相色谱系统下以碱性(胺类)和酸性(酚类)化合物为溶质对该固定相的正相色谱行为进行考察。结果表明该键合相在正相色谱模式下对碱性化合物具有良好的分离选择性,而酸性化合物在流动相中加入改性剂乙酸的条件下也实现了较好的分离。研究表明,正相模式下该键合相的保留机理存在着氢键、静电吸引及π-π等相互作用机制。A microcolumn liquid chromatographic system was set up by packing imidazole functionalized silica stationary phase into capillary,and organic compounds including basic and acidic analytes were separated under normal-HPLC mobile phase using the packed column（0.25 mm × 150 mm,2 μm）.The chromatographic performance and retention mechanism of the stationary phase under this chromatographic condition were studied.Results showed that the stationary phase had an obvious priority for the efficient separation of basic compound under normal phase chromatographic condition,and acidic compound could also be well separated when a little of modified acetic acids were added to the mobile phase.The study of mechanism indicated that the stationary phase provided various action mechanisms for different analytes under this chromatographic condition,such as those of hydrogen bonding,electrostatic force and π-π interactions.

关 键 词：咪唑键合硅胶固定相 正相作用 微柱液相色谱 

分 类 号：O657.72[理学—分析化学]