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机构地区:[1]六盘水职业技术学院,贵州六盘水553000 [2]贵州大学动物科学学院,贵州贵阳550025
出 处:《贵州农业科学》2010年第6期162-164,共3页Guizhou Agricultural Sciences
基 金:六盘水市科技计划项目"贵州黑山羊高繁殖率真候选基因ESR研究"(52020-2008-1-02)
摘 要:通过对贵州黑山羊LHβ基因序列进行分析,研究贵州黑山羊LHβ基因的多态性及其与产仔数间的关系。结果表明,在贵州黑山羊30只母羊的LHβ基因832 bp核苷酸系列中,共发现2个变异位点,分别位于138 bp和240 bp处,均为同义变异,其中240 bp处碱基由C变成T,且变异前后的平均产仔数相同,而在138 bp处的碱基由G变成C,形成AA和AB 2种基因型,AB基因型的羊比AA基因型的平均产仔数多产1.02只,且差异显著(P<0.01)。说明,LHβ基因138 bp位点的多态性与贵州黑山羊繁殖性能之间可能存在相关性。The LHβ gene sequence of Guizhou black goat was analyzed to study LHβ gene polymorphism and the relationship between LHβ gene polymorphism and litter size.The results showed that there were two variation loci at 138bp and 240bp in 832bp of LHβ gene of 30 female Guizhou black goats and both of the variation were synonymous mutation.The C at 240bp was replaced by T and there was no difference in average litter size before and after variation.The G at 138bp was replaced by C and two genotypes of AA and AB were formed.The average litter size of Guizhou black goats with AB genotype was 1.02 goats higher than that with AA genotype and there was significant difference in average litter size between two genotypes,which may indicate that there is correlation between 138bp polymorphism of LHβ gene and reproduction performance in Guizhou black goat.
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