贵州非洲菊主栽品种工厂化育苗技术研究  

Study on Large-scale Seedling Culture of Major Gerbera jamesonii Cultivars in Guizhou

作　　者：李英[1] 郑思乡[1] 

机构地区：[1]贵州省园艺研究所,贵州贵阳550006

出　　处：《贵州农业科学》2010年第6期190-192,195,共4页Guizhou Agricultural Sciences

基　　金：贵州省农业科学院博士基金项目"贵州省非洲菊优质种苗规范化生产技术(GAP)研究与推广"[黔农科院人才启动项目2008008]

摘　　要：为生产提供优质的非洲菊种苗,以非洲菊桔红品种大零88和红色品种热带草原的幼蕾为外植体,选用MS为基本培养基,附加激素6-BA、KT和NAA,研究了不同激素浓度组合对外植体不定芽分化、增殖培养和生根培养的影响。结果表明：诱导芽分化时,大零88以MS＋6-BA6 mg/L＋KT 4 mg/L＋NAA0.1 mg/L的效果最佳;热带草原以MS＋6-BA9 mg/L＋KT 4 mg/L＋NAA0.1 mg/L的效果最佳。增殖培养中,大零88以MS＋6-BA0.3~0.5 mg/L＋KT0.2~0.4 mg/L＋NAA0.01~0.05 mg/L为佳;热带草原以MS＋6-BA0.2~0.4 mg/L＋NAA0.01 mg/L为佳。生根培养大零88以MS＋NAA0.4 mg/L＋IAA0.1 mg/L的生根率达100%,热带草原以MS＋NAA0.3~0.4 mg/L＋IAA0.1~0.2 mg/L的生根培养基最好。The young buds of Daling 88 and Tropical plain（two Gerbera jamesonii cultivars） were cultured on MS with different combination of 6-BA,KT and NAA to study effects of different hormone combination on its adventitious bud differentiation,multiplication and rooting for produce a large number of quality seedlings.The results showed that the optimum media for adventitious bud differentiation of Daling 88 and Tropical plain were MS＋6mg/L 6-BA＋4mg/L KT＋0.1mg/L NAA and MS＋9mg/L 6-BA ＋4mg/L KT ＋0.1mg/L NAA,the optimum media for multiplication culture of Daling 88 and Tropical plain were MS＋0.3～0.5mg/L 6-BA＋0.2～0.4mg/L KT＋0.01～0.05mg/L NAA and MS＋0.2～0.4mg/L 6-BA ＋0.01mg/L NAA,and the optimum media for bud rooting of Daling 88 and Tropical plain were MS＋0.4mg/L NAA ＋ 0.1mg/L IAA and MS ＋0.3～0.4mg/L NAA ＋ 0.1～0.2mg/L IAA respectively.

关 键 词：非洲菊 组织培养 种苗生产 

分 类 号：S682.19[农业科学—观赏园艺]