猪T细胞受体β链基因的克隆及生物信息学分析  

Cloning and bioinformatics analysis of porcine T cell receptor beta chain gene

作　　者：李玩生[1,2] 刘磊[1] 冯海燕[2] 房永祥[2] 景志忠[2] 

机构地区：[1]甘肃农业大学动物医学院,甘肃兰州730070 [2]中国农业科学院兰州兽医研究所,家畜疫病病原生物学国家重点实验室,甘肃省动物寄生虫病重点实验室,甘肃兰州730046

出　　处：《甘肃农业大学学报》2010年第3期1-6,13,共7页Journal of Gansu Agricultural University

基　　金：国家高新技术"863"项目(2006AA10A203);国家支撑计划项目(2007BAD40B03)

摘　　要：以GenBank上登载的猪的T细胞受体β(pTCR-β)基因为参考序列,用RT-PCR法从猪的外周血淋巴细胞中克隆了TCR-β链基因,并进行生物信息学分析.结果表明:pTCR-β基因含有一个完整的开放阅读框架(ORF),大小为849bp,编码283个氨基酸,且含有一段27个氨基酸的信号肽序列,与参考序列相比,在核苷酸序列上的同源性为80.4%,在氨基酸序列上的同源性为70.3%;生物信息学结构预测发现2个结构域,一个为IG结构域,由第32-138位共107个氨基酸残基组成;另一个为IG-LIKE结构域,由第164-211位共48个氨基酸残基组成.Based on the gene of porcine T cell receptor beta chain（AB079529）from the GenBank database, T cell receptor beta chain gene was cloned by reverse transcription-polymerase chain reaction （RT-PCR） from porcine peripheral-blood lymphocyte （PBL）.Sequence analysis showed that pTCR-β gene was 849 bp in length and encoded 283 amino acids,including a signal peptide of 27 amino acids,comparing with the reference sequence pTCR-β gene shared 80.4 % homology with the published sequence of nucleotide and shared 70.3 % homology of amino acids;two domain had been found by the structure prediction of bioinformatics,one was IG domain that contained 107 amino acids with position of 32-138,another was IG_like domain that contained 48 amino acids with position of 164-211.The pTCR-β gene was cloned successfully,which provided a foundation for the study of structure and function of beta chain.

关 键 词：猪TCR-β链基因 基因克隆 生物信息学 结构预测 

分 类 号：S828[农业科学—畜牧学]