口蹄疫病毒株AF72 P1的结构分析  

Structural analysis of P1 from a foot-and-mouth disease virus strain AF72

作　　者：陈启伟[1,2] 王永录[2] 杨孝朴[1] 

机构地区：[1]甘肃农业大学动物医学院,甘肃兰州730070 [2]中国农业科学院兰州兽医研究所,家畜疫病病原生物学国家重点实验室,农业部畜禽病毒学重点开放实验室,甘肃兰州730046

出　　处：《甘肃农业大学学报》2010年第3期7-13,共7页Journal of Gansu Agricultural University

基　　金：国家支撑计划项目(2006BAD06A06)

摘　　要：对口蹄疫病毒AF72株P1结构蛋白基因进行了扩增、克隆,并采用DNAStar Protean软件对P1蛋白的二级结构、可塑性、亲水性、表面可及性及抗原指数等参数进行分析,综合预测其B细胞表位分布.结果表明:FMDV AF72株P1基因全长2211bp,包含完整的开放阅读框,编码737个氨基酸,其中,VP1长639bp,编码213个氨基酸,VP2长654bp,编码218个氨基酸,VP3长663bp,编码221个氨基酸,VP4长255bp,编码85个氨基酸.P1结构蛋白的二级结构较为复杂,含有较多的β片层结构和转角结构,VP1、VP2和VP3上均有多个区域为B细胞优势表位,VP4上也有少量的潜在B细胞表位.The DNA of P1 gene of Foot-and-mouth disease virus AF72 was amplified, cloned, and then sequenced. Amino acid sequence of P1 on secondary structure, f1exibility, hydrophilicity, accessibility and antigenicity were analyzed using DNAStar Protean software, with the B cell epitopes to be predicted. The results showed that the P1 region of FMDV AF72 was 2 211 bp in length and coded for a polypeptide of 737 amino acids, including complete open reading frame.The structural protein of VP1, VP2, VP3, VP4 gene consisted of 639, 654, 663,255 bp and coded for 213, 218, 221, 85 amino acids respectively. The secondary structure of P1 region was relatively complicated consisting of more β sheet regions and turn regions. There were predominant B cell epitopes in VP1,VP2,VP3 and a small amounts in VP4. Comparing with the epitopes that had been published, the predict results had a high reliability. This study would be helpful for identification of B cell epitopes for structural protein of FMDV AF72 strain using experimental methods and research of reverse vaccine of FMDV.

关 键 词：口蹄疫病毒 结构蛋白 B细胞表位 

分 类 号：S852.659.6[农业科学—基础兽医学]