Chi和Bar基因双价植物表达载体的构建及转基因烟草的初步检测  

Construction of plant expression vector containing Chi and Bar genes and introduction them into tobacco

作　　者：吴文俊[1] 贾小霞[1] 张金文[1] 王汉宁[1] 

机构地区：[1]甘肃农业大学农学院,甘肃兰州730070

出　　处：《甘肃农业大学学报》2010年第3期56-59,65,共5页Journal of Gansu Agricultural University

基　　金：国家科技支撑计划课题(2007BAD52B08)

摘　　要：构建了单子叶植物双价表达载体pBI121-Ubi-Bar/Chi,其中含有木霉几丁质酶基因Chi和抗除草剂Bar基因,均由玉米Ubi启动子驱动.以烟草无菌苗叶片为受体,用农杆菌介导法将目的基因转入烟草中;以Bar基因作为选择标记,PPT为选择剂进行筛选,获得了一定数量的转基因植株.结果表明:经PCR分析,初步确定目的基因已经整合到烟草基因组中.Monocotyledon plant bivalent expression vector was constructed as pBI121-Ubi-Bar/Chi which included Trichoderma chitinase gene Chi and resistant herbicide Bar gene that were started by Ubi promoter of maize.The target genes were transferred directly to tobacco by Agrobacterium mediated method,and the receptor was leaf of tobaco aseptic seedling.Some trans-genetic plants were obtained by selecting with the PPT as selector, and Bar gene was selecting marker. The target genes had been integrated to tobacco genome preliminarily through PCR analysis.

关 键 词：几丁质酶基因 抗除草剂基因 载体构建 烟草 转化 

分 类 号：S572[农业科学—烟草工业]