乙肝表面抗原在微球表面的吸附行为、活性和结构变化  

Adsorption Behavior, Bioactivity and Structure of HBsAg on Surface of PLA Microspheres

作　　者：李娜 王连艳[1] 吴有斌[1,2] 曾烨婧[1,3] 马光辉[1] 苏志国[1] 

机构地区：[1]中国科学院过程工程研究所生化工程国家重点实验室,北京100190 [2]中国科学院研究生院,北京100049 [3]北京化工大学生命科学与技术学院,北京100029

出　　处：《过程工程学报》2010年第3期559-567,共9页The Chinese Journal of Process Engineering

基　　金：国家自然科学青年基金资助项目(编号:50703043);国家高技术研究发展计划(863)基金资助项目(编号:2007AA09Z439)

摘　　要：采用快速膜乳化技术结合溶剂挥发法制备了尺寸均一的聚乳酸(PLA)微球,平均粒径为800nm左右,并采用PLA微球对乙肝表面抗原(HBsAg)进行了吸附研究,考察了pH值、盐浓度、微球用量、HBsAg浓度及吸附温度对HBsAg吸附率、活性和结构的影响.结果表明,在pH6.0的磷酸缓冲溶液中,NaCl浓度为20g/L、微球用量为20mg/mL、HBsAg浓度为10μg/mL、吸附温度为37℃的条件下,HBsAg吸附率可达60%左右.4℃下,pH值为8.0及NaCl浓度为1g/L、微球用量为2mg/mL及HBsAg浓度为300μg/mL时,HBsAg活性保留可达98%以上.Relatively uniform-sized poly（lactic acid） （PLA） microspheres with the average particle size of 800 nm were successfully prepared by premix membrane emulsification combined with solvent evaporation method. The effects of pH value, salt concentration, amount of PLA microspheres, HBsAg concentration and adsorption temperature on the adsorption efficiency, bioactivity retention and structure of HBsAg were systemically investigated. The results showed that the adsorption efficiency of HBsAg on the surface of PLA microspheres could reach more than 60% under the conditions of pH 6.0 phosphate buffer, 20 g/L NaCl concentration, 20 mg/mL PLA microspheres, 10 μg/mL HBsAg concentration and 37℃ adsorption temperature. The bioactivity retention of HBsAg exceeded 98% under 4℃ with the following conditions： pH value of phosphate buffer at 8.0, concentration of NaCl 1 g/L, amount of microspheres 2 mg/mL and initial HBsAg concentration 300 μg/mL.

关 键 词：聚乳酸微球 乙肝表面抗原 吸附 生物活性 结构 

分 类 号：R944.5[医药卫生—药剂学]