疏水相互作用与亲水相互作用组合色谱纯化缩宫素  

Purification of Oxytocin by Combining Hydrophobic Interaction Chromatography and Hydrophilic Interaction Chromatography

作　　者：张晓鸥[1] 宋茂谦[2] 王长海[1] Jan-Christer Janson 顾铭[2] 

机构地区：[1]大连理工大学环境与生命学院生物科学与工程系,辽宁大连116024 [2]中国科学院过程工程研究所生化工程国家重点实验室,北京100190 [3]Department of Physical and Analytical Chemistry,Uppsala Biomedical Centre,Uppsala University

出　　处：《过程工程学报》2010年第3期598-602,共5页The Chinese Journal of Process Engineering

基　　金：国家自然科学基金资助项目(编号:20606032;20636010;50773083);生化工程国家重点实验室开放基金资助项目(编号:SKLBE-KF002)

摘　　要：将疏水相互作用色谱与亲水相互作用色谱结合,建立了用于化学合成缩宫素粗品分离纯化的两步纯化工艺.先以纯水为流动相,采用Nylon6疏水相互作用色谱对粗品进行脱盐与富集,后以Superose12亲水相互作用色谱进行进一步纯化,两步纯化后,峰面积纯度达93.5%,提高了5.96倍,总回收率为50.1%,与用C18为介质的传统两步反相色谱纯化的结果相当.本方法有机溶剂用量仅为传统反相色谱的1/7,且两种介质都能耐受极端pH值清洗条件,重复利用率高.A two-step process involving hydrophobic interaction chromatography and hydrophilic interaction chromatography was developed to purify chemically synthetic oxytocin out of economic and environmental consideration. First, Nylon 6 hydrophobic interaction chromatography was performed using water as mobile phase for desalting and enrichment. And Superose 12 hydrophilic interaction chromatography was performed for further purification. By this two-step purification process, the purity of oxytocin was increased up to 93.5% （5.96 folds purification） with final recovery rate of 50.1%, which was comparable with the results obtained from traditional two-step RPLC purification process with C18 medium. This two-step purification process consumed less organic solvent, which meant lower cost. Nylon 6 and Superose 12 media were chemically stable under extreme pH cleaning conditions and easily reproduced.

关 键 词：聚己内酰胺 琼脂糖凝胶 疏水相互作用色谱 亲水相互作用色谱 缩宫素 纯化 

分 类 号：TQ460.64[化学工程—制药化工] TQ028.8