大鼠部分肝脏缺血再灌坏死模型的优化及磁共振评价方法  

Optimization of Rat Model on Ischemic Reperfused Liver Necrosis and Characterization with Multiparametric Magnetic Resonance Imagine

作　　者：张健[1] 孙胜禄[1] 孔铭[1] 姚楠[1] 荆梅[1] 黄德健[1] 孙自平[2] 倪以成[3] 

机构地区：[1]江苏省中医药研究院,江苏南京210028 [2]山东医学科学院放射医学研究所,山东济南250062 [3]比利时鲁汶大学

出　　处：《药物生物技术》2010年第3期228-231,共4页Pharmaceutical Biotechnology

基　　金：江苏省国际合作项目(BZ2009097);江苏省中医局项目(LZ09064)

摘　　要：该研究旨在优化大鼠部分肝脏缺血再灌坏死实验模型,探讨磁共振影像技术评价该模型的实验方法。30只大鼠随机分为3组,阻断肝右叶血供,分别于1、2、3 h后放开阻断,再灌24 h后处死,经TTC组织化学染色测定肝叶坏死体积;取10只大鼠按照优化后方法,制成部分肝脏缺血再灌坏死模型,用磁共振成像方法对该模型评价。结果显示阻断1 h右肝坏死不完全,阻断2、3 h右肝彻底坏死(>90%),表明大鼠右肝供血阻断2 h,再灌流24 h即可成功制作该模型。坏死肝叶在磁共振T2 WI(P<0.01)和DWI(P<0.01)上呈高信号,T1 WI成像上呈等或略高信号(P<0.05),CE-T1 WI呈高信号(P<0.05),可以用于坏死模型的评价。To optimize the rat model of ischemic reperfused liver necrosis （IRLN） and to characterize it by muhiparametric magnetic resonance imagine （MRI）, IRLN was induced in 30 rats, which were divided into 3 groups, by occluding hepatic inflow to the right liver lobe for 1,2, 3 h respectively and reperfusing for 24 h. Then the rats were sacrificed and the＇ necrotic volumes of the right livers were measured by TTC staining. According to the conditions finally selected, reperfused partial liver infarc tion （RPIA） was induced in 10 rats by occluding hepatic inflow to the right liver lobe for 2 h and reperfusing for 24 h. MRI was performed at a 1.5T clinical scanner to obtain T1-weight （T1WI）, T2-weight （T2WI）, contrast-enhanced （CE） TlWI and diffusion weighted imaging （DWI）. Rats were sacrificed for histomorphology. The necrotic right liver lobe reached over 90% of the volume by 2 h occlusion of hepatic inflow to the right liver lobe. The infarct lobe was conspicuous from normal liver with the highest signal intensity on T2WI （P〈0.01） and DWI （P〈0.01）. The signal intensity of infarct liver was slightly higher than that of normal liver on TlWI and CE T1WI （after gadolinium injection）. MRI find ings were verified by histomorphology and the RPLI in rats could be made by occluding hepatic inflow to the right liver for 2 h and reperfusion for 24 h. It could be characterized by muhiparametric MRI in T2WI and DWI.

关 键 词：动物模型 肝脏 缺血再灌 磁共振 

分 类 号：R965.1[医药卫生—药理学]