均匀设计法优化桦木醇超声波辅助提取工艺  

Optimization of Ultrasound-assisted Extraction of Betulin Using Uniform Design

作　　者：丁为民[1,2] 周丹[3] 于涛[1] 黄靖姝[1] 阎秀峰[1] 王洋[1] 

机构地区：[1]东北林业大学生命科学学院,林木遗传育种与生物技术教育部重点实验室,黑龙江哈尔滨150040 [2]哈尔滨理工大学化学与环境工程学院,黑龙江哈尔滨150080 [3]黑龙江省森林植物园,黑龙江哈尔滨150040

出　　处：《林产化学与工业》2010年第3期78-82,共5页Chemistry and Industry of Forest Products

基　　金：引进国际先进林业技术项目(2006-4-119);东北林业大学优秀青年教师创新基金项目(无编号);中央高校基本科研业务费专项资金资助(DL09EA02)

摘　　要：以95%(体积分数,下同)的乙醇为溶剂,利用均匀设计法优化了超声波辅助提取白桦树皮中桦木醇的工艺,考察了各因素对桦木醇提取率的影响,并以高效液相色谱法测定了萃取物中桦木醇的含量。结果表明,在超声波功率400 W、提取时间70 min、提取温度70℃、液固比40∶1(mL∶g)的条件下,超声波辅助提取桦木醇提取率可达到83.43%±1.89%,纯度71.20%±0.74%。与传统回流提取法相比,超声波辅助提取法提取时间短、提取率高,具有一定的应用前景。用5%的碳酸钠水溶液对原料进行预处理,可减少酸性杂质对桦木醇提取的干扰。White birch（Betula platyphylla Suk.） bark,one of the important biomaterial resources,is the remains after cutting and processing of the tree.Ultrasound-assisted extraction of betulin from white birch bark using uniform design was optimized.The effects of ultrasound power,extraction temperature,extraction time and liquid-solid ratio on betulin yield was discussed,and betulin content was determined by HPLC.Betulin yield could reach up to 83.43%±1.89% with the purity of 71.20%±0.74% under the conditions of ultrasound power 400W,extraction time 70min,extraction temperature 70℃,and liquid-solid ratio 40∶1（mL∶g）.Comparing with the traditional reflux extraction,shorter extraction time and better yield were achieved by ultrasound-assisted extraction.Through pre-treatment with 5% sodium carbonate aqueous solution the interference of acidic impurities on the extraction of betulin could be reduced.

关 键 词：桦木醇 白桦树皮 均匀设计 超声波辅助提取 高效液相色谱 

分 类 号：TQ351[化学工程] S789.1[农业科学—林学]