利用分子标记辅助选择聚合Xa4、Xa23和Pi9基因  

Pyramiding Xa4,Xa23 and Pi9 genes by molecular marker-assisted selection

作　　者：梁海福[1] 阎勇[1] 秦钢[1] 唐梅[1] 莫海玲[1] 于松保[1] 

机构地区：[1]广西农业科学院水稻研究所,南宁530007

出　　处：《广西农业科学》2010年第5期408-411,共4页Guangxi Agricultural Sciences

基　　金：广西青年科学基金项目(桂科青07288027);广西农业科学院科技发展基金项目(2006002)

摘　　要：利用常规回交育种结合分子标记辅助选择技术,将高抗水稻白叶枯病Xa4和Xa23基因、广谱高抗稻瘟病Pi9基因聚合到同一株系中,获得了三基因聚合的纯合株系。选用广西具有代表性的13个稻瘟病菌株、白叶枯病广致病型菌系P6和中国7个流行菌系对杂交后代进行人工接种鉴定,结果表明,聚合了Xa4、Xa23和Pi9基因的株系09-371、09-375能同时抗白叶枯病和稻瘟病,对白叶枯病抗谱和抗性水平与供体材料W7相当,对稻瘟病的抗性与供体亲本75-1-127水平相当。Xa4、Xa23和Pi9三基因纯合株系可以作为水稻育种的多抗供体材料。In the present study, rice donor material W7 for bacterial blight and 75-1-127 for rice blast were used and back-cross breeding in combination with molecular marker-assisted selection was used to pyramid high rice bacterial blight resistance genes Xa4 and Xa23 and broad-spectrum rice blast resistance gene P/9 in a rice strain. By analysis and screening with molecular marker MP12, C189 and PB8, 2 three-gene （Xa4, Xa23 and Pi9） pyramiding homozygous rice strains 09-371 and 09-375 were obtained in F4. By artificial inoculation with 13 prevailing Guangxi strains of rice blast, broad pathogenicity bacterial blight strain P6, and 7 prevailing national strains of bacterial blight, three-gene pyramiding strains 09-371 and 09-375 were found to be resistant to both rice bacterial blight as well as blast at the same time. The level and spectrum of bacterial blight resistance in three-gene pyramiding strains were similar to the donor parent W7 of Xa4 and Xa23, and those of rice blast in three-gene pyramiding strains were similar to the donor parent 75-1-127 of Pi9. The three-gene pyramiding homozygous strains may be used as multiple-resistant donor parent in rice breeding programs.

关 键 词：水稻 白叶枯病 稻瘟病 Xa4 XA23 Pi9 分子标记辅助选择 

分 类 号：S511.035.3[农业科学—作物学]