甘蔗内生固氮菌液浸种对甘蔗生长前期氮代谢相关酶活性的影响  

Effects of sett-soaking with sugarcane endophytic diazotroph solution on N metabolism related enzymes activities at the early growth stage of sugarcane

作　　者：欧阳雪庆[1] 罗霆[1,2,3,4] 杨丽涛[1,4] 李杨瑞[1,3,4] 

机构地区：[1]广西大学广西亚热带生物资源保护利用重点实验室,南宁530004 [2]广西农业科学院经济作物研究所,南宁530007 [3]广西作物遗传改良生物技术重点开放实验室,南宁530007 [4]中国农业科学院甘蔗研究中心,南宁530007

出　　处：《广西农业科学》2010年第5期416-418,共3页Guangxi Agricultural Sciences

基　　金：国家自然科学基金项目(30660085);国家科技支撑计划项目(2007BAD30B03);广西科技攻关重点项目(桂科攻0782004-3);广西科技攻关项目(桂科能0815011);现代农业产业技术体系建设专项资金项目(nycytx-024-01-15)

摘　　要：为研究甘蔗内生固氮菌液浸种对甘蔗生长前期氮代谢相关酶活性的影响,分别用从甘蔗品种桂糖28号、新台糖22号、B8中分离获得的3种内生固氮菌及其混合液对甘蔗浸种处理,并测定与氮代谢相关酶活性指标。结果表明,接种甘蔗内生固氮菌对甘蔗叶片硝酸还原酶活性和谷氨酰胺合成酶活性具有普遍而明显的促进作用,而对谷草转氨酶与谷丙转氨酶的影响则因甘蔗品种差异表现出不同效果;巴西品种B8的谷草转氨酶与谷丙转氨酶活性显著提高,但固氮菌对本地品种桂糖28号和新台糖22号的谷草转氨酶与谷丙转氨酶活性影响不大。The present experiment has been conducted to study the effects of sett-soaking with sugarcane endophytic diazotroph solution on N metabolism related enzyme activities at the early growth stage of sugarcane. Sugarcane seedlings （setts） were soaked with four endophytic diazotrophs solutions extracted from sugarcane varieties Guitang 28, ROC22 and B8 and their mixture, and activities of enzymes related to N metabolism were assayed. The results showed that nitrate re- ductase and glutamine synthase activity in sugarcane leaves significantly enhanced in plants treated with sugarcane endo- phytic diazotrophs ~olution, while its effects on the activities of glutamic-oxaloacetic transminase and glutamic-pyruvic transminase differed in various treatments, and which were significantly higher in variety B8 compared to the local varieties GT28 and ROC22.

关 键 词：甘蔗 内生固氮菌 硝酸还原酶 谷草转氨酶 谷丙转氨酶 谷氨酰胺合成酶 酶活性 

分 类 号：S566.101[农业科学—作物学]