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作 者:王其刚[1] 张颢[1] 蹇洪英[1] 邱显钦[1] 李树发[1] 唐开学[1]
机构地区:[1]云南省农业科学院花卉研究所,云南省花卉育种重点实验室,云南昆明650205
出 处:《江西农业大学学报》2010年第3期458-461,共4页Acta Agriculturae Universitatis Jiangxiensis
基 金:国家“863”计划子课题(2006AA100109);国家科技支撑计划子课题(2006BAD01A1805);云南省科技攻关项目(2006NG14)
摘 要:采用红四唑(TTC)染色法测定切花月季新品种云粉、云玫的花粉活力和寿命,联苯胺—过氧化氢测定柱头的可授性,显微观察其柱头的形态、颜色变化,结合田间人工授粉试验研究其育性。结果表明:(1)23℃干燥条件下,散粉4 d内花粉活力保持在50%以上,保存10 d时,花粉活力降至6%以下。(2)云粉柱头活性持续时间为3 d以上,云玫不超过2 d,强可授性持续时间云粉远远高于云玫。(3)田间试验,云粉作母本结实率均高于70%,作父本时结实率差异极显著;云玫作亲本时,结实率不超过16%,甚至不结实。可为我国自主知识产权月季品种应用于杂交育种提供科学依据。Pollen viability and life-span of cut Chinese roses varieties Yunfen and Yunmei were evaluated by TTC.The stigma receptivity was estimated by benzidine-H2O2.The development of stigma's modality and color was observed by microscope.Hand-pollination in the field was also adopted to study the fertility in Chinese rose.The results showed:1.The pollen germination rate was higher than 50% in four days after shedding pollens and reduced to six percent at the tenth day.2.The stigma of Yunfen kept high receptivity for more than 3 days,while Yunmei lasted less than 2 days.The best receptible time of Yunfen was much longer than that of Yunfen.3 In field crossing,the hip setting was higher than 70% when Yunfen was the female parent.The hip setting differed much from the male parent.As for Yunmei,there were few hips gotten when it was used as either female or male parent.The result of this experiment will provide a scientific basis for varieties of roses which have been protected in China to be used in cross-breeding.
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