白鹭源禽Ⅰ型副粘病毒L基因的克隆与序列分析  

Cloning and sequence analysis of L gene of avian paramyxovirus 1 isolated from egret

作　　者：何奇松[1] 孙翔翔[1] 易春华[1] 蒋家霞[1] 付薇[2] 马琳[2] 熊毅[2] 

机构地区：[1]广西大学动物科学技术学院,南宁530005 [2]广西动物疫病预防控制中心,南宁530001

出　　处：《广西农业科学》2010年第5期481-484,共4页Guangxi Agricultural Sciences

基　　金：广西科技攻关项目(桂科攻0719004-3G)

摘　　要：根据GenBank中已发表的鹅副粘病毒GPV-SF02株全基因组序列设计5对引物,采用RT-PCR扩增出白鹭源禽I型副粘病毒(W13株)L基因的LA(1121 bp)、LB(1481 bp)、LC(1439 bp)、LD(1476 bp)、LE(1608 bp)5个片段,用DNA Star软件比较分析后进行拼接,获得长约6760 bp包含有L基因全长的核苷酸序列,而W13株L基因的mRNA全长为6704 bp、开放阅读框(ORF)为6615个碱基、编码2204个氨基酸。氨基酸同源性分析表明:W13株L基因与鹅源SF02、ZJl、NA-1株及鸡源Guangxi7株的同源性均超过97.0%;而核苷酸同源性分析表明:W13株L基因与鹅源SF02、ZJl、NA-1株及鸡源Guangxi7株的同源性均超过93.0%。可见,W13株与鹅源SF02、ZJl、NA-1株及鸡源Guangxi7株的亲缘关系较近。In the present study, strain W13 derived from egret was used and five pairs of primers were designed from genome sequence of paramyxovirus GPV-SF02 strain of goose （GenBank）. Five fragments of L gene of avian paramyxovirus 1 strain W13 derived from egret, viz., LA （1121 bp）, LB （1481 bp）, LC （1439 bp）, LD （1476 bp） and LE （1608 bp） were amplified by RT-PCR. After comparative analysis using DNA Star software, five fragments were joined and 6760 bp of nucleotide sequence containing whole length of L gene was obtained. It was found that whole length of mRNA of L gene from strain W13 was 6704 bp, and the open reading frame contained 6615 basic groups and coded 2204 amino acids. The homology analysis of amino acid and nucleotide showed that L gene from strain W13 had more than 97.0% and 93.0% homology to strains SF02, ZJ1, NA-1 derived from goose and Guangxi7 derived from chicken, respectively. The results revealed that strain W13 had closer relationship to strain SF02, ZJI, NA-1 and Guangxi7.

关 键 词：禽I型副粘病毒 L基因 克隆 序列分析 

分 类 号：S852.659.5[农业科学—基础兽医学]