运用ISSR分析蜂螨对雄蜂及雄蜂蛹的选择性寄生机制  

Studies on the Selective Parasitism Mechanism of Mites to Drone and Drone-Pupa by ISSR

作　　者：刘益波[1] 吴小波[1] 颜伟玉[1] 曾志将[1] 

机构地区：[1]江西农业大学蜜蜂研究所,江西南昌330045

出　　处：《江西农业大学学报》2010年第3期581-584,共4页Acta Agriculturae Universitatis Jiangxiensis

基　　金：引进国际先进农业科学技术948项目(2006-G19-2);国家蜂产业技术体系资助项目(nycytx-43-kxj15)

摘　　要：以被大蜂螨(Varroa destructor)寄生的意大利蜜蜂(Apis mellifera ligustica)为实验材料,分别采集有螨雄蜂、无螨雄蜂、有螨雄蜂蛹和无螨雄蜂蛹各40只,运用ISSR(inter-simple sequence repeat)标记对各样品进行DNA扩增,通过fine-dox3型凝胶成像系统分析各条带的片段大小,计算各特异性条带在有螨与无螨样品中的概率。结果表明:有些特异性条带在有螨与无螨样品中的概率差异极显著(P<0.01),由此推测,这些条带可能与蜂螨的选择性寄生有关。Experiments were conducted with Apis mellifera ligustica,which was infected by Varroa destructor.40 drones with vorroa mites,non-mite drones,pupas with mites and non-mite pupas,individually were collected.The length of the bands was analyzed by fine-dox3 chemidoc,it imaging system after PCR amplification and the probability of the peculiar-bands in the samples with and without mites was calculated.The results showed that the probability of some peculiar-bands in the samples with and without mites was significantly different（P0.01）,It is hence conclueded that these bands have samething to do with the selective parasitism of mites.

关 键 词：大蜂螨 ISSR 特异性条带 

分 类 号：S895.32[农业科学—特种经济动物饲养]