HPLC测定楤木叶中绿原酸的含量  

HPLC DETERMINATION OF CHLOROGENIC ACID IN ARALIA CHINENSIS LEAF

作　　者：何兵[1] 田吉[1] 肖顺汉[1] 

机构地区：[1]泸州医学院药学院,四川泸州646000

出　　处：《泸州医学院学报》2010年第3期229-231,共3页Journal of Luzhou Medical College

基　　金：四川省教育厅重点项目(No.2005A076)

摘　　要：目的:建立HPLC测定楤木叶中绿原酸含量的方法。方法:色谱柱为Dikma Kromasil C18柱(250mm×4.6mm,5μm);流动相为乙腈-0.1%磷酸溶液(12∶88),检测波长326nm。结果:绿原酸在0.654～6.536μg范围内具有良好的线性关系(r=0.9999),平均回收率为98.73%,RSD为0.78%。结论:该方法简便、快速、准确、具有良好的重复性和回收率,可作为楤木叶中绿原酸含量测定方法。Objective：To establish an HPLC method for determination of chlorogenic acid in Aralia chinensis leaf.Methods：The separation was carried out on a Dikma Kromasil C18 column （250 mm×4.6mm,5μm）.The mobile phase was acetonitrile-0.1% phosphoric acid （12∶88）.The detective wavelength was set at 326 nm.Results： The calibration curve showed good linearity over the range of 0.654～6.536μg （r=0.9999）. The average recovery was 98.73% with RSD 0.78%. Conclusion：This method is simple,rapid,accurate and with good repeatability and recovery,it can be used as a quantitative analysis method for Aralia chinensis leaf.

关 键 词：HPLC 楤木叶 绿原酸 

分 类 号：R927.2[医药卫生—药学]