铜胁迫对狭叶香蒲生长及生理特性的影响  

Effect of Cu^(2+) stress on the growth and physiological properties of Typha angustifolia

作　　者：赵艳 徐迎春[2] 柴翠翠[2] 周燕[2] 

机构地区：[1]南京溧水县特殊教育学校,江苏溧水211200 [2]南京农业大学园艺学院,南京210095

出　　处：《广西植物》2010年第3期367-372,共6页Guihaia

基　　金：山东农业大学作物生物学国家重点实验室资助项目~~

摘　　要：采用水培法,研究不同浓度Cu^(2+)胁迫对狭叶香蒲生长及生理特性的影响。结果表明:狭叶香蒲叶宽、株高的增长量及整株干物质累积量在较低浓度Cu^(2+)处理时均未受影响,此后随Cu^(2+)浓度升高显著下降。Cu^(2+)浓度为o～30 mg·L^(-1)时,叶绿素含量显著上升,此后随Cu^(2+)浓度增加其含量显著下降。在Cu^(2+)浓度0～5 mg·L^(-1)范围内根系活力显著上升,此后随Cu^(2+)浓度升高则大幅下降。根系和叶片SOD、POD、CAT、活性随Cu^(2+)浓度增加均呈先显著升高后显著降低的趋势,根系SOD、POD活性在30 mg·L^(-1)时出现最大值,叶片SOD、POD活性在55 mg·L^(-1)时出现最大值,根系和叶片CAT活性均在80 mg·L^(-1)时出现最大值;同一Cu^(2+)浓度下,根系SOD、POD、CAT活性明显高于叶片,说明根系比叶片对Cu^(2+)胁迫反应更敏感。从根系和叶片SOD/POD、SOD/CAT的比值变化上看出,在Cu^(2+)为1 mg·L^(-1)和5 mg·L^(-1)时起主要保护作用的酶是SOD、POD,后来CAT起到主要作用。MDA含量自Cu^(2+)浓度为30 mg·L^(-1)时开始持续上升。说明在Cu^(2+)为30～55 mg·L^(-1)时狭叶香蒲表现为积极的生理响应。Effects of Cu^2＋ stress on physiological properties and growth of Typha angustifolia were studied by hydroponic culture method. The results showed that the leaf width,plant height and the dry weight accumulation were not obviously affected at the low Cu^2＋ concentration,but decreased with the increasing Cu^2＋ concentration. The content of chlorophyll increased among 0-30 mg · L^-1 Cu^2＋ and decreased obviously with the increasing of Cu^2＋ concentration. Root activity increased among 0-5 mg· L^-1 Cu^2＋ and also decreased apparently afterwards with the increasing of Cu^2＋ concentration.. The activities of SOD, POD and CAT increased with the increasing of Cu^2＋ concentration and decreased clearly afterwards. The highest value of SOD, POD of roots appeared at 30 mg · L^-1 Cu^2＋ , while the peak values of in leaves were at 55 mg· L^-1 Cu^2＋. The activity of CAT reached to the highest at 80 mg· L^-1. The activities of SOD, POD and CAT of root were higher than leaves apparently under the same Cu^2＋ concentra- tion stress,it was suggested that sensitivity of roots to Cu^2＋ concentrations were higher than that of leaves. The activities of SOD, POD played the main role on protecting T. angustifolia under 1- 5 mg· L^-1Cu^2＋ stress, while CAT played the main role afterwards. The content of MDA reached to the high point at 30 mg· L^-1 Cu^2＋ and increased afterwards with the increasing of Cu^2＋ concentration. It was suggested that T. angustifolia had an active physiological response under 30-55 mg · L^-1 Cu^2＋ stress.

关 键 词：狭叶香蒲 CU^2+胁迫 生长 生理特性 

分 类 号：Q945[生物学—植物学]