低剂量辐射对荷S180肉瘤小鼠肿瘤组织MMP-2和TIMP-2表达影响  

EFFECT OF LOW DOSE RADIATION ON TUMOR EXPRESSIONS OF MMP-2 AND TIMP-2 IN S180 SARCOMA-BEARING MICE

作　　者：张艳敏[1] 于洪升[1] 

机构地区：[1]青岛大学医学院附属医院肿瘤科,山东青岛266003

出　　处：《齐鲁医学杂志》2010年第3期214-216,共3页Medical Journal of Qilu

摘　　要：目的探讨低剂量辐射对荷S180肉瘤小鼠肿瘤组织中基质金属蛋白酶2(MMP-2)、金属蛋白酶组织抑制因子2(TIMP-2)表达的影响。方法昆明种小鼠皮下接种S180肉瘤细胞,待肿瘤形成后将小鼠随机分为假照组(N组,40只)和低剂量辐射组(LDR组,40只),N组予以无射线假照射,LDR组给予一次性75 mGy的60Co射线全身照射。N组于假照射后12 h处死,LDR组分别于照射后12、24、48、72 h处死。分别取肿瘤称质量,免疫组化染色检测肿瘤组织中MMP-2、TIMP-2表达情况。结果与N组相比,75 mGy射线全身照射后小鼠肿瘤生长缓慢(t=2.19,P<0.05)。LDR组小鼠低剂量照射后不同时间TIMP-2表达与N组比较,差异无统计学意义(P>0.05);MMP-2的表达在照射后24、48 h较N组明显下降,差异均有显著性(u=2.15、2.04,P<0.05)。结论低剂量全身照射可以明显抑制肿瘤生长,降低MMP-2的表达,从而抑制肿瘤的浸润和转移。Objective To investigate the effect of low dose radiation（LDR）on expressions of MMP-2 and TIMP-2 in S180 sarcoma-bearing mice. Methods A tumor model of S180 sarcoma-bearing mice was created by subcutaneous inoculation of S180 sarcoma cells into Kunming strain mice.The mice were then divided into sham-radiation group（N group,40 mice） and low dose radiation group（LDR group,40 mice）.The mice in LDR group were given single dose of 75 mGy for whole-body γ-ray radiation;those in N group were radiated with no ray,and sacrificed 12 h after sham-radiation;those in LDR group were sacrificed 12 h（LDR-12 h group）,24 h（LDR-24 h group）,48 h（LDR-48 h group） and 72 h（LDR-72 h group） after radiation.Tumor weight was determined,and the expressions of MMP-2 and TIMP-2 detected by immunohistochemical method. Results Compared with N group,whole-body exposure to 75 mGy γ-ray in LDR group significantly slowed down the tumor growth of mice（t=2.19,P0.05）.After low dose radiation,the expression of TIMP-2 at different time points in LDR group were not significantly different from those observed in N group（P0.05）.The MMP-2 expression was significantly reduced in LDR-24 h group（u=2.15,P0.05） and LDR-48 h group（u=2.04,P0.05） compared with that of N group. Conclusion Low dose whole-body radiation could significantly suppress the growth of tumor and reduce the expression of MMP-2,therefore inhibit the infiltration and metastasis of tumors.

关 键 词：放射治疗剂量 肉瘤180 明胶酶A 金属蛋白酶2组织抑制剂 

分 类 号：R815.2[医药卫生—放射医学]