实验性精索静脉曲张大鼠睾丸氧化应激水平的检测  

DETECTION OF TESTICULAR OXIDATIVE STRESS LEVEL IN EXPERIMENTAL VARICOCELE IN RATS

作　　者：贾月峰[1] 王新生[1] 马征[1] 李延江[1] 孙立江[1] 刘忠强[2] 李华琴[2] 王沛涛[2] 

机构地区：[1]青岛大学医学院附属医院泌尿外科,山东青岛266003 [2]青岛大学医学院附属医院低温医学男科,山东青岛266003

出　　处：《齐鲁医学杂志》2010年第3期251-253,共3页Medical Journal of Qilu

摘　　要：目的测定青春期精索静脉曲张（VC）大鼠睾丸组织中氧化应激水平,探讨VC致不育的机制。方法将40只雄性青春期Wistar大鼠随机分为VC 8周组、VC 12周组（各12只）和相应的假手术对照组（各8只）,通过部分结扎左肾静脉建立实验性大鼠VC模型,对照组只游离左肾静脉不结扎。分别于术后8、12周解剖动物,测左侧精索静脉最大直径;比色法测睾丸组织内过氧化氢（H2O2）、过氧化氢酶（CAT）、超氧化物歧化酶（SOD）和总抗氧化能力（T-AOC）水平。结果成功建立了VC模型。VC 8周组和12周组大鼠左侧精索静脉均明显扩张（t=7.19、6.86,P〈0.01）,双侧睾丸组织内H2O2含量较对照组明显增加（t=2.31~4.78,P〈0.05、0.01）,与右侧相比左侧增加更明显（t=2.56、3.15,P〈0.05）,并且左侧VC 12周组比VC 8周组增加更明显（t=2.16,P〈0.05）。VC 8周组和VC 12周组左侧CAT活力与对照组比较显著降低（t=2.23、3.03,P〈0.05、0.01）,右侧数值有下降趋势但差异无显著性（t=0.95、1.41,P〉0.05）。VC 8周组和VC 12周组双侧SOD活力和T-AOC与对照组比较均明显降低（t=3.29~4.72,P〈0.01）,并且VC 12周组比VC 8周组降低更明显（t=2.80、3.38,P〈0.05、0.01）,T-AOC左侧睾丸比右侧下降更明显（t=11.66、7.54,P〈0.01）。结论 VC可引起双侧睾丸H2O2增加,CAT活力、SOD活力以及T-AOC下降,导致睾丸组织内氧化应激系统失衡。Objective To detect the level of oxidative stress in testis of experimental adolescent varicocele in rats and study its mechanism of infertility. Methods A model of varicocele was created in 24 adolescent male Wistar rats by partially ligating the left renal veins,which were equally randomized to group A and group B（varicocele for 8 and 12 weeks,respectively）.Eight rats underwent sham-operation and another eight served as a control group received only a dissection of the left renal vein.The animals were dissected at 8 and 12 weeks after surgery and the maximum diameter of the left spermatic veins measured,levels of H2O2,CAT,SOD and T-AOC of the testes checked biochemically. Results A model of varicocele was successfully created.Compared with the corresponding control groups,the diameter of the left spermatic veins were significantly enlarged（t=7.19,6.86;P0.01）;the levels of H2O2 in both testes of groups A and B significantly increased（t=2.31,P0.05;t=3.59,4.78,P0.01）,that of the left side increased more（t=2.56,3.15;P0.05） and that in group B was much higher than that in group A（t=2.16,P0.05）.Activity of CAT of left testes in groups A and B more reduced than that of the controls（t=2.23,P0.05;t=3.03,P0.01）,while that of the right testis had a downturn but with no statistical significance（t=0.95,1.41;P0.05）.Levels of SOD and T-AOC of the both-side in both varicocele groups were lower than that of the control（t=3.29-4.72,P0.01）,and more remarkable reduction was seen in group B than in group A（t=2.80,P0.05;t=3.38,P0.01）,the T-AOC of the left testis was lower than that of the right（t=11.66,7.54;P0.01）. Conclusion Varicocele induces the increase of H2O2 content of both testes,decrease of vigor of CAT,SOD and T-AOC,resulting in disbalance of oxidative stress response system in testis.

关 键 词：精索静脉曲张 睾丸 氧化性应激 大鼠 Wistar 

分 类 号：R697.24[医药卫生—泌尿科学]