HPLC法测定紫花前胡中紫花前胡苷的含量  

Determination of Nodakenin in Peucedanum decursivum.(Miq.) Maxim by HPLC

作　　者：周婵[1] 罗建光[1] 孔令义[1] 

机构地区：[1]中国药科大学天然药物化学教研室,南京210009

出　　处：《药学与临床研究》2010年第3期253-255,共3页Pharmaceutical and Clinical Research

基　　金：<中国药典>2010版一部品种质量标准修订项目(yz-246);国家自然科学基金重点项目(30830116);国家重大科技专项(20092X09502-011)

摘　　要：目的:建立HPLC法测定中药紫花前胡中紫花前胡苷含量的方法,为其质量控制提供依据。方法:采用反相高效液相色谱法,Agilent Zorbax SB-C18色谱柱(4.6mm×250mm,5μm),流动相为甲醇-水(40:60),流速为1mL·min-1,检测波长334nm,柱温40℃。结果:紫花前胡苷在此色谱条件下获得良好分离,线性范围为25.0～300.0ng(r2=0.9999),平均回收率为98.6%,RSD1.8%。结论:首次建立了两相系统HPLC法测定紫花前胡药材中紫花前胡苷含量的方法,该方法准确,可靠,对紫花前胡药材及其制剂的质量控制有较重要意义,已被2010版《中国药典》采纳。Objective：To develop an HPLC method to determine the contents of nodakenin in Peucedanum decursivum,and provide scientific basis for its quality control and preparation.Method：The analysis was carried out on an Agilent Zorbax SB-C18 column eluted with methanol and water as mobile phases.The detection wavelength was 334 nm,and the flow rate was 1.0mL·min-1.Result：The nodakenin had a good linear range of 25.0～300.0ng（r2=0.999 9）,with the average recovery and RSD of 98.6% and 1.8%,respectively.Conclusion：This method for quantification of nodakenin in Peucedanum decursivum is simple,accurate and reliable and can be used for the its quality control of and preparations.It has been adopted by the China Pharmacopeia of 2010 edition.

关 键 词：紫花前胡 紫花前胡苷 HPLC 两相系统 

分 类 号：R927.2[医药卫生—药学]