小鼠肠道优势菌群失衡肠黏液sIgA、黏液素的变化  

Changes of intestinal sIgA and mucin in mice with intestinal dysbacteria

作　　者：李晓芳[1] 刘洋[1] 文姝[1] 唐立[1] 

机构地区：[1]大连医科大学微生态学教研室,辽宁大连116044

出　　处：《中国微生态学杂志》2010年第6期485-488,共4页Chinese Journal of Microecology

基　　金：国家自然科学基金(30670045);国家"973"科学计划项目(2007CB513006);国家"863"科学计划项目(2007A10Z356)

摘　　要：目的研究肠道菌群失衡对小鼠肠黏膜机械屏障的影响,探讨优势菌群与肠道黏膜屏障的相互作用机制。方法利用ELISA法检测肠黏膜sIgA含量变化,RT-PCR技术及免疫组化法检测肠黏膜上皮细胞Mucin2、Mucin3和防御素mRNA转录产物的变化。结果菌群失衡小鼠小肠段肠黏膜sIgA水平下降,重度菌群失衡组与正常组比较下降显著(P<0.05),与轻度菌群失衡组相比下降明显。菌群失衡小鼠上皮细胞中的Mucin2、Mucin3和防御素mRNA转录产物与正常小鼠相比明显增高,在杯状细胞胞浆内Mucin2蛋白阳性表达增强,且重度菌群失衡组与轻度菌群失衡组小鼠比较,Mucin2、Mucin3和防御素增高明显。结论菌群失衡可导致肠黏膜sIgA分泌量随菌群失衡程度呈下降趋势,同时引起黏液层中黏液素和防御素分泌量增加。Objective To observe the changes of intestinal physical barrier of dysbacteria mice,and explore the interaction of intestinal dominant flora and intestinal mucosal immunity. Method The sIgA in intestinal mucosa were detected by ELISA; Mucin2,mucin3 and defensin were detected by RT-PCR and immunohistochemical method.Result Compared with the normal group,the mucosal sIgA levels were decreased in dysbacteria groups; the sIgA level of severe-dysbacteria group was significantly lower than that of normal group （P0.05） and obviously lower than that of mild-dysbacteria group. The levels of mucin2,mucin3 and defensin of dysbacteria groups were higher than those of normal group,with those of the severe-dysbacteria group higher than those of mild-dysbacteria group. Conclusion Antibiotics such as ceftriaxone sodium can inhibit and kill intestinal dominant flora; In dysbacteria mice,the secretory sIgA volume reduces according to the severity of dysbacteria,while intestinal mucin and defensin levels increase.

关 键 词：菌群失衡 SIGA 黏液素 防御素 

分 类 号：R378.992[医药卫生—病原生物学]