白香丹胶囊对经前期综合征肝气逆证大鼠下丘脑GABA_ARβ_2亚基分布及表达的影响  

Effect of Baixiangdan capsule on location and expression of GABA_A receptor subunit β_2 in hypothalamus of premenstrual syndrome model rats with liver-Qi invasion

作　　者：田园[1] 张惠云[1] 

机构地区：[1]山东中医药大学天然药物实验室,山东济南250355

出　　处：《山东中医杂志》2010年第7期479-481,共3页Shandong Journal of Traditional Chinese Medicine

基　　金：国家重点基础研究发展规划"973"计划项目(2006CB504804);国家自然科学基金资助项目(30930110)

摘　　要：目的:研究白香丹胶囊对经前期综合征(PMS)肝气逆证模型大鼠下丘脑γ-氨基丁酸A受体(GABAAR)β2亚基蛋白分布和蛋白、mRNA表达的影响及PMS肝气逆证发病中枢机制。方法:以免疫荧光技术(IF)、蛋白印迹技术(Western blot)、半定量逆转录多聚合酶链式反应(半定量RT-PCR)依次检测大鼠下丘脑GABAARβ2亚基蛋白的分布模式,蛋白、mRNA的表达水平。结果:与正常组比较,肝气逆组大鼠下丘脑GABAARβ2亚基主要分布在下丘脑组织的边缘,蛋白、mRNA表达水平显著升高;造模同时给予白香丹胶囊后,受体蛋白在下丘脑中均匀分布而趋于正常,蛋白、mRNA表达水平有不同程度的降低。结论:GABAARβ2亚基蛋白分布异常,蛋白、mRNA表达上调可能是PMS肝气逆证发病中枢机制之一,白香丹胶囊可以对上述异常变化起纠正作用。Objective:To study the effect of Baixiangdan capsule on location of protein and expression of protein and mRNA of GABAA receptor subunit β2(GABAARβ2) in hypothalamus of premenstrual syndrome(PMS) model rats with liver-Qi invasion and its the mechanism of PMS with liver-Qi invasion Central nervous systerm.Methods:The location of GABAARβ2 protein and ex-pression of protein and mRNA in hypothalamus of rats were detected by immunofluorescence(IF),Western bolt and semi-quanti-tative RT-PCR in turn.Results:Compared with the normal group,receptor protein distributed primarily in the fringe of hypotha-lamus,and the expression of protein and mRNA of GABAARβ2 were increased significantly in the liver-Qi invasion group rats.After given Baixiangdan capsule,the location of receptor protein tended to normal,and the expression of mRNA and protein were decreased in differnt tendency.Conclusion:The location of GABAARβ2 protein are abnormal.One of the mechanism of PMS with liver-Qi invasion may be related with the abnormal location and high expression of protein and mRNA of GABAARβ2 in hypothalamus.In addition,Baixiangdan capsule has modulatory effect on above changes.

关 键 词：白香丹胶囊 PMS肝气逆证 GABAARβ2亚基 IF Western BLOT 半定量RT-PCR 

分 类 号：R285.5[医药卫生—中药学]