鸡新城疫病毒ND-xx08株HN基因的克隆及分析  

Cloning and analysis of HN gene of Newcastle disease virus ND-xx08 isolate

作　　者：贾文科[1] 王选年[2] 许兰菊[1] 银梅[2] 岳峰[2] 宋涛[2] 

机构地区：[1]河南农业大学牧医工程学院,河南郑州450002 [2]河南科技学院动物科学学院,河南新乡453003

出　　处：《河南农业大学学报》2010年第3期300-302,310,共4页Journal of Henan Agricultural University

基　　金：河南省高校科技创新团队支持计划(2008IRTSTHN001)

摘　　要：为克隆并分析新城疫病毒ND-xx08株HN基因,将其分离、纯化、培养,提取其病毒的基因组RNA,RT-PCR扩增获得与HN基因预期大小一致的DNA片段,克隆到pMD18-T载体,经EcoR I和Hind Ⅲ进行双酶切鉴定和测序鉴定.ND-xx08 HN基因的序列长度为1 716 bp,共编码571个氨基酸.与已发表的24株NDV HN序列的同源性分析表明,其核苷酸序列的同源性在80.0%~98.4%,氨基酸同源性在87.2%~98.2%.研究结果表明,ND-xx08具有强毒株的特性.In order to clone and analyze of HN gene of Newcastle disease virus ND-xx08 isolate,it was separated,purified and cultured to obtain the virus genome RNA.DNA fragments was acquired by RT-PCR amplification.The DNA fragment was then cloned into pMD-18 vector for sequence and double enzyme digested by EcoR I and Hind III identification.The sequencing results showed the length of the HN gene is 1 716 bp,encoding 571 amino acids.The analysis showed that the nucleotide sequence homology was 80.0% to 98.4% and amino acid homology was 87.2% to 98.2% compared with 24 published NDV sequences.This research result indicated that ND-xx08 isolate had veolgenic strain characteristics.

关 键 词：鸡新城疫病毒 ND-xx08株 HN基因克隆 

分 类 号：S855.3[农业科学—临床兽医学]