实验时间对老年Sprague-Dawley大鼠认知行为的影响  

Experiment-time affects the cognitive behavior in aged Sprague-Dawley rats

作　　者：邓世宁[1] 司文[1] 孙丽娟[1] 曾庆文[1] 曹晓华[1] 

机构地区：[1]华东师范大学脑功能基因组学教育部重点实验室,上海市脑功能基因组学重点实验室,上海200062

出　　处：《生理学报》2010年第3期231-236,共6页Acta Physiologica Sinica

基　　金：supported by the National Natural Science Foundation of China(No.30670682);Shanghai Key Laboratory of Brain Functional Genomics Project(No.44501645)

摘　　要：为了探明老年Sprague-Dawley（SD）大鼠的学习记忆能力是否受到实验时间的影响,我们将老年SD大鼠随机分为两组：一组在上午（8：00~11：00）进行行为学实验（上午组,AM组）,另一组则在下午（15：00~18：00）进行行为学实验（下午组,PM组）。结果显示,在开场实验中,PM组的活动量显著高于AM组;此外,在Morris水迷宫任务的训练阶段,PM组比AM组表现出更好的空间学习记忆能力,而且在检测（probe-test）过程中,PM组也表现出更强的空间记忆能力;而在新异个体识别（social discrimination）行为实验中,两组大鼠没有表现出显著性差异。以上结果表明,实验时间是老年SD大鼠在水迷宫任务中表现的影响因素,但并不是新异个体识别任务的影响因素。提示实验时间对老年大鼠不同的学习记忆任务具有不同的影响。In order to investigate if the experiment-time affects cognitive performance in aged rats in different learning and memory behavioral tests, the aged Sprague-Dawley （SD） rats were divided into two groups randomly and subjected to open field test, Morris water maze test and social discrimination test. The experiment of one group was conducted in 8：00 11：00 AM （AM group）, and the other group in 15：00 18：00 PM （PM group）. PM group exhibited higher locomotor activity than AM group in the open field test. Compared with AM group rats, PM group rats had significantly shorter swimming distance and escape latency to find the platform in Morris water maze training session, but no significant difference in the swimming velocity was observed between the two groups. And in probe-test, PM group spent more time in target quadrant than AM group. These results revealed that PM group showed better spatial learning and memory abilities than AM group. However, there was no significant difference between the two groups in social investigation index in social discrimination test. These results show that experiment-time may affect cognitive performance in Morris water maze but not in social discrimination task in aged rats. And the results indicate that experiment-time affects cognitive performance of aged rats selectively in different learning and memory behavioral tasks.

关 键 词：老年大鼠 学习记忆 开场实验 MORRIS水迷宫 新异个体识别 

分 类 号：R33[医药卫生—人体生理学]