应用双抗体夹心ELISA定量检测鼠疫F1单克隆抗体IgG含量  

Quantitative Detection for Monoclonal Antibody IgG to Plague F1 Antigen with Double-antibody Sandwich ELISA

作　　者：杜春红[1] 唐雪[1] 杨光璨[1] 宋志忠[1] 

机构地区：[1]云南省地方病防治所,云南大理671000

出　　处：《地方病通报》2010年第3期8-10,共3页Endemic Diseases Bulletin

基　　金：云南省科技攻关项目(2007CA010)

摘　　要：目的建立一种用于定量检测鼠疫单克隆抗体样品中IgG含量的方法 ,以更好地指导杂交瘤细胞的筛选和抗体的生产、纯化。方法采用羊抗鼠IgG为包被抗体、辣根过氧化物酶标记的羊抗鼠IgG为标记抗体,纯化鼠IgG作参考品,制作标准曲线,测定未知样品中的IgG含量。结果该方法与BCA蛋白浓度测定法测得的结果基本一致,且重复性好,特异性强,灵敏度高。结论双抗体夹心ELISA法可用于测定鼠疫F1单克隆抗体样品中的IgG含量。Objective To establish a quantitative detection method of content of monoclonal antibody IgG to Yersinia pestis for the purpose of well guiding screening of hybridoma cells and production and purification of monoclonal antibody.Methods Goat anti-mouse IgG and its labeling with horseradish peroxidase were used as the coated antibody and second antibody.Purified mouse IgG was used as reference materials to produce the standard curve,which determined the IgG content of unknown samples.Results The protein concentration detected by double-antibody sandwich ELISA was basically consistent with BCA assay,with good repeatability,specificity and high sensitivity.Conclusions Double-antibody sandwich ELISA method can be used to measure the IgG content of F1 monoclonal antibodies to Yersinia pestis.

关 键 词：鼠疫 单克隆抗体 夹心ELISA法 IGG 

分 类 号：R378.61[医药卫生—病原生物学] R446.6[医药卫生—基础医学]