高效液相色谱法测定人血清中普卢利沙星活性代谢物浓度  

HPLC used determine serum concentrations of active metabolite of prulifloxacin

作　　者：邹静[1] 童荣生[1] 何林[1] 董芸[2] 陈璐[1] 肖洪涛[1] 

机构地区：[1]四川省医学科学院.四川省人民医院药剂科,四川成都610041 [2]四川省医学科学院.四川省人民医院急诊科,四川成都610041

出　　处：《中国医院药学杂志》2010年第12期1015-1018,共4页Chinese Journal of Hospital Pharmacy

摘　　要：目的：建立HPLC快速测定人血清中普卢利沙星活性代谢物（NM394）浓度。方法：以氟罗沙星为内标，样品经酸化沉淀蛋白后，上清液先后分别以3mI。正己烷和2H止醋酸乙酯进行反洗后进样。分析柱Diamonsil Platisil C18（150mm×4．6mm，5μm），保护柱Dikma EasyGuard C18（8mm×4mm，5μm），柱温35℃；流动相为乙腈－0．02mol·L^-1磷酸二氢钾（pH2．7，含0．5％三乙胺）（10：40．5），流速1mL·min^-2，检测波长275nm。结果：NM394在10～5000μg·L^-1范围内线性关系良好（r^2=0．9997），最低定量限为10μg·L^-1。日内、日间精密度均小于6％，方法学回收率为96．31％～102．49％。结论：本方法快速、准确、灵敏、选择性高，适用于普卢利沙星人体药动学研究。OBJECTIVE To develop an HPLC detection for the quantification of active metabolite of prulifloxacin （NM394） in human serum. METHODS Fleroxacin was used as internal standard （IS）, after protein precipitation and a simple two-step back-wash procedure, NM394 and 1S were separated on a C18 column with a mobile phase containing acetonitrile 0. 02 mol· L^-1 KH2 PO4 （pH 2. 7, containing 0. 5 % of triethylamine） （10： 40. 5） at a flow rate of 1 mL·min^-1. The analytes were detected at 275 nm. RESULTS Calibration curves were linear （r^2 = 0. 999 7） from 10-5 000μg·L^-1. Both intra-day and inter-day precisions were less than 6%. Methodology recovery was 96. 31%-102.49%. Limit of quantification for NM394 was 10μg·L^-1. CONCLUSION HPLC described herein had been successfully applied for human pharmacokinetic studies, which offers advantages of specificity,quickness and higher sensitivity over the previously reported methods.

关 键 词：普卢利沙星 NM394 氟罗沙星 高效液相色谱法 紫外检测 

分 类 号：R927.2[医药卫生—药学]