HPLC测定千柏鼻炎片中对羟基桂皮酸和金丝桃苷的含量  

Simultaneous Determination of p-Hydroxycinnamic Acid and Hyperoside in Qianbai Biyan Tablets by HPLC

作　　者：谭道鹏[1] 侴桂新[2,3,4,5,6] 王峥涛 

机构地区：[1]中国药科大学中药学院,南京210038 [2]上海中医药大学中药研究所 [3]中药标准化教育部重点实验室 [4]中药新资源与质量标准综合评价国家中医药管理局重点研究室 [5]上海市复方中药重点实验室,上海201203 [6]上海中药标准化研究中心,上海201203

出　　处：《中国现代应用药学》2010年第6期532-534,共3页Chinese Journal of Modern Applied Pharmacy

基　　金：“十一五”国家科技支撑计划(2006BAI14B01)

摘　　要：目的建立千柏鼻炎片中对羟基桂皮酸和金丝桃苷的含量测定方法。方法应用高效液相色谱法测定,色谱柱为Dikma Diamonsil-C18(4.6mm×250mm,5μm);流动相为乙腈-0.2%冰醋酸(15∶85);流速1.0mL·min-1;检测波长310nm(0～25min),360nm(25～35min)。结果对羟基桂皮酸和金丝桃苷的线性范围分别为0.014088～1.174μg(r=0.9999),0.00955～1.91μg(r=0.9999);平均加样回收率分别为101.17%(RSD=1.35%),100.89%(RSD=3.80%)。结论该方法简便易行、准确、重复性好,可用于千柏鼻炎片的质量控制。OBJECTIVE To establish a high performance liquid chromatographic method for the determination of p-hydroxycinnamic acid and hyperoside in Qianbai Biyan tablets.METHODS The chromatographic separation was achieved on a Dikma Diamonsil-C18（250 mm×4.6 mm,5 μm） column at a variable wavelength from 310 nm（0-25 min） to 360 nm（25-35 min） using an isocratic mobile phase consisted of a mixture of 0.2% aqueous acetic acid and acetonitrile in the ratio of 85：15 at a flow rate of 1.0 mL·min^-1.RESULTS Linearity relationships were 0.014 088-1.174 μg（r=0.999 9）,0.009 55-1.91 μg（r=0.999 9）,respectively;the average recoveries were 101.17%（RSD=1.35%） and 100.89%（RSD=3.80%）.CONCLUSION The results showed that the method is simple,accurate and repeatable and it is suitable for the determination of p-hydroxycinnamic acid and hyperoside in Qianbai Biyan tablets.

关 键 词：千柏鼻炎片 千里光 对羟基桂皮酸 金丝桃苷 

分 类 号：R917.101[医药卫生—药物分析学]