21个兰花品种的延长RAPD(ERAPD)和PCR-RFLP分析  

Molecular Characterization of Chinese Cymbidium Based on Extended RAPD (ERAPD) and PCR-RFLP Markers

作　　者：蹇黎[1,2] 武海[1] 张以忠[2] 余丹凤[2] 秦小军[2] 朱利泉[1] 

机构地区：[1]西南大学农学与生物技术学院,重庆400715 [2]毕节学院环境与生命科学系,贵州毕节551700

出　　处：《中国农学通报》2010年第14期232-237,共6页Chinese Agricultural Science Bulletin

基　　金：贵州省教育厅自然科学研究项目(2007082)

摘　　要：为明确中国兰花品种间在DNA水平上的遗传多样性和亲缘关系。应用ERAPD(延长随机引物扩增)、叶绿体和线粒体基因组PCR-RFLP对21个中国兰花品种进行分子鉴定。结果表明,RAPD分析中共有8个(10%)引物和88条(83.81%)多态性带纹;ERAPD分析中能获得1条2.5kbRAPD特异性片段;叶绿体基因组(cpDNA)的PCR-RFLP分析中有4个(66.67%)引物和12个(25%)引物-酶组合;线粒体基因组中(mtDNA)的PCR-RFLP分析中有2个(33.33%)引物和1个(4%)引物-酶组合能揭示材料间遗传差异。3种分子标记均能揭示品种间在DNA水平上存在遗传差异,而且遗传学分类与形态学分类基本吻合。In order to reveal the genetic variations and relationship of 21 Chinese Cymbidium cultivars.21 cultivars from 7 cymbidium species were analyzed by ERAPD,cpDNA and mtDNA PCR-RFLP markers.The results showed that 88 polymorphic bands were observed in 8 out of 80 random primers（10% ） in RAPD analyses,the 2.5 Kb RAPD fragment were obtained in ERAPD analysis,genetic difference were revealed in 4 primers（66.67% ） and 12 primer-enzyme combinations（25% ） by cpDNA PCR-RFLP analysis,2 primers （33.33%） and 1 primer-enzyme combinations（4%） by mtDNA PCR-RFLP analysis.The results indicated that these molecular markers can detect the genetic difference in 21 Chinese Cymbidium cultivars.

关 键 词：兰花 叶绿体基因组(cpDNA) PCR-RFLP 延长随机引物扩增DNA(ERAPD) 线粒体基因组(mtDNA) 

分 类 号：S682.31[农业科学—观赏园艺]