高效液相色谱法测定乳病消片中淫羊藿苷的含量  

Determination of Icariin in Rubingxiao Tablets by HPLC

作　　者：马云海[1] 李红卫[1] 李涛[2] 

机构地区：[1]兰州大学第二医院,兰州730030 [2]兰州大学药学院

出　　处：《中国药师》2010年第7期986-988,共3页China Pharmacist

摘　　要：目的:建立乳病消片中淫羊藿苷的含量测定方法。方法:色谱柱:Hypersil BDS C_(18)(150 mm×4.6 mm,5μm);流动相:甲醇-水(54∶46);柱温:30℃;流速:1 ml·min^(-1);检测波长:270 nm。结果:淫羊藿苷在4.04～72.72μg·ml^(-1)浓度范围内与峰面积呈良好的线性关系,r=0.9999,回收率为98.2%,RSD为1.6%。结论:本法简便、准确,可以用于制剂质量控制。Objective： To establish an HPLC method for determination of icariin in rubingxiao tablets. Method： The Hypersll BDS-C18（ 150 mm ×4.6 mm,5μm） column was used ,the mobile phase consisted of methanol： water （54： 46） at the flow rate of 1 ml · min^- 1 ;the wavelength was set at 270 nm and the column remperature was at 30 ℃. Result： There was a good linear relationship over the range of 4.04-72. 72 μg-m1-1 for icariin （r =0. 999 9）. The recorery was 98. 2% （RSD = 1.6% ）. Conclusion： This method is simple and accurate, it can be applied to the determination of the tablets.

关 键 词：乳病消片 高效液相色谱法 淫羊藿苷 含量测定 

分 类 号：R927.2[医药卫生—药学]