吴茱萸不同炮制品中绿原酸的含量比较  

Comparison the Contents of Chlorogenic Acid in Different Processed Products of Evoia rutaecarp(Juss.) Benth

作　　者：温慧玲 欧阳胜[2] 谢一辉[2] 

机构地区：[1]江西青峰药业有限公司,江西赣州341000 [2]江西中医学院药学系

出　　处：《中国药师》2010年第7期988-990,共3页China Pharmacist

基　　金："十一五"国家科技支撑计划(编号:2006BAI06A07-2)

摘　　要：目的:用反相高效液相色谱法测定吴茱萸及其炮制品中绿原酸的含量,探讨不同炮制方法对于吴茱萸中绿原酸含量的影响。方法:采用Diamonsil C_(18)色谱柱(250 mm×4.6 mm,5μm);流动相:乙腈-0.4%磷酸水溶液等度洗脱;流速:1.0 ml·min^(-1):检测波长:327 nm;柱温:25℃。结果:吴茱萸不同炮制品绿原酸的含量较吴茱萸生品均有不同程度的降低。结论:浸泡制炮制方法比浸润制炮制方法对于绿原酸含量的降低作用更大。Objective： To explore the different processing methods on the content changes of ehlorogenic acid in Evodia and pro- cessed products by RP-HPLC. Method： Dimonsil C18column （250 mm ×4.6 mm,5 μm） was used. The mobile phase was acetonitrile-0.4% phosphate water solution with gradient elution. The flow rate was 1 ml·min 1. The detection wavelength was at 327 μm and the column temperature was 25 ℃ Result： The contents of chlorogenic acid in Evodia and processed products was different, which in the processed products were lower than Evodia. Conclusion： The contents of chlorogenie acid with immersion system is lower than infiltration system.

关 键 词：吴莱萸 绿原酸 含量测定 RP—HPLC 

分 类 号：R927.2[医药卫生—药学]