流式细胞仪检测恶性胸腔积液中T淋巴细胞亚群变化  

CHANGE OF T-LYMPHOCYTE SUBSETS IN MALIGNANT PLEURAL EFFUSION EXAMINED BY FLOW CYTOMETRY

作　　者：郑玉兰[1] 吴金如[1] 李向阳[1] 黄远东[1] 莫扬[1] 

机构地区：[1]湖北省襄樊市中心医院呼吸内科,襄樊441021

出　　处：《现代预防医学》2010年第14期2720-2721,共2页Modern Preventive Medicine

摘　　要：[目的]探讨胸腔积液T淋巴细胞亚群检测对恶性病变的诊断价值。[方法]用流式细胞仪对28例肺癌和23例良性病变的患者胸腔积液T淋巴细胞亚群分析。[结果]中央型肺癌组胸腔积液CD3+CD4+(46.21±11.57)%、CD3+CD8+(52.33±10.78)%和CD4+/CD8+(0.97±0.36),周围型肺癌组胸腔积液中胸腔积液CD3+CD4+(42.35±9.59)%、CD3+CD8+(57.85±9.76)%和CD4+/CD8+(0.81±0.41),良性病变组胸腔积液CD3+CD4+(63.45±7.31)%、CD3+CD8+(32.54±7.12)%和CD4+/CD8+(2.14±0.31),前两组差异无统计学意义(P﹥0.05)。前两组显著低于良性病变组,P﹤0.05。[结论]胸腔积液T淋巴细胞亚群的改变对良恶性病变的鉴别诊断有重要的意义。[Objective] To evaluate the diagnostic value of flow cytometric T-lymphocyte subsets in malignant pleural effusion.[Methods] The T-lymphocyte subsets of pleural effusion from 28 patients with lung cancer and 23 patients with benign disorder were analyzed by flow cytometry.[Results] The percentage of CD3^＋CD4^＋ was（46.21 ± 11.57）%,CD3^＋CD8^＋ was（52.33 ± 10.78）% and CD3^＋CD4^＋/CD3^＋CD8^＋ was（0.97 ± 0.36）in BALF with central lung cancer,and the percentage of CD3^＋CD4^＋ was（42.35 ± 9.59）%,CD3^＋CD8^＋ was（57.85 ± 9.76）% and CD3^＋CD4^＋/CD3^＋CD8^＋ was（0.81 ± 0.41）in pleural effusion with peripheral lung cancer,which were significantly lower than those of CD3^＋CD4^＋（63.45 ± 7.31）%,CD3^＋CD8^＋（32.54 ± 7.12）% and CD3^＋CD4^＋/CD3^＋CD8^＋（2.14 ± 0.31）in pleural effusion with benign disease,and there was no significant difference between central lung cancer and peripheral lung cancer.[Conclusion] Flow cytometric examination of T-lymphocyte subsets in pleural effusion is effective in differentiating benign and malignant disorder.

关 键 词：胸腔积液 流式细胞仪 T淋巴细胞亚群 

分 类 号：R561[医药卫生—呼吸系统]