葡萄糖-6-磷酸异构酶抗原对类风湿关节炎的诊断价值  

Diagnostic value of glucose-6-phosphate isomerase antigen in patients with rheumatoid arthritis

作　　者：尹志华[1] 叶志中[1] 罗秀霞[1] 曹智君[1] 董慧娟[1] 

机构地区：[1]深圳市第四人民医院香蜜湖风湿病分院广东医学院深圳风湿病研究所,广东深圳518040

出　　处：《现代生物医学进展》2010年第10期1911-1912,1931,共3页Progress in Modern Biomedicine

基　　金：深圳医学重点学科建设资助项目(2005C10)

摘　　要：目的:通过比较类风湿关节炎(Rheumatoid arthritis,RA)患者、非RA风湿病患者及健康对照者血清中葡萄糖-6-磷酸异构酶(glucose-6-phosphate isomerase,GPI)抗原的阳性率来探讨GPI对RA的诊断意义,并探讨GPI,抗CCP抗体和RF联合应用对RA的诊断价值。方法:对110例RA患者、223例非RA风湿病患者和55例健康对照者共388份血清标本进行了检测。GPI抗原和抗CCP抗体采用ELISA方法,RF采用免疫比浊法定量检测。结果:RA组、非RA风湿病组和健康对照组血清中的GPI抗原阳性率分别为83.64%,30.04%和20.00%,RA组阳性率显著高于其它组(P<0.01)。GPI抗原对RA诊断的敏感性和特异性分别为83.64%和71.58%。GPI和抗CCP抗体联合检测的敏感性和特异性分别为90.91%和71.22%,GPI和RF联合检测的敏感性和特异性分别为92.73%和61.87%,如果三者同时检测,其敏感性和特异性分别为94.55%和60.43%。结论:RA患者的血清GPI阳性率明显高于其它自身免疫性疾病患者和健康对照者。GPI抗原对RA具有诊断价值,联合检测GPI、抗CCP抗体和RF可以提高RA诊断的敏感性。Objective：To compare GPI antigen prevalence in RA patients,other autoimmune diseases＇patients and healthy controls in a Chinese population,ant to evaluate the diagnostic values of GPI antigen combined with anti-cyclic citrullinated peptide（anti-CCP） and rheumatoid factors（RF） in Chinese patients with RA.Methods：We studied 388 serum samples from 110 patients with RA,223 with other autoimmune diseases and 55 healthy controls.GPI antigen and anti-CCP was assessed by enzyme-linked immunosorbent assay.RF was measured by a liquid-phase immunoprecipitation assay with nephelometric end-point detection.Results：The positive rates of GPI were 83.64%,30.04% and 20.00% in RA group,other autoimmune diseases group and healthy group,respectively.The positive rate of GPI in RA group was significantly higher than that in the other groups（p〈0.01）.The sensitivity and specificity rates of GPI for diagnosis of RA were 83.64% and 71.58% respectively.The sensitivity and specificity rates of GPI combined anti-CCP for diagnosis of RA were 90.91% and 71.22%,while GPI combined RF the rates were 92.73% and 61.87% respectively.If the three indexes combined together,the sensitivity and specificity rates for diagnosis of RA were 94.55% and 60.43%.Conclusions：The positive rate of GPI in RA group is significantly higher than in other groups.GPI antigen is valuable for diagnosis of RA.Combined detection of GPI,anti-CCP and RF could increase the diagnostic sensitivity rate of RA.

关 键 词：类风湿关节炎 葡萄糖-6-磷酸异构酶 抗胍氨酸环肽抗体 类风湿因子 

分 类 号：R593.22[医药卫生—内科学]