卵泡刺激素受体短肽的表达及纯化  

Expression and purification of follicle-stimulating hormone receptor

作　　者：袁栎[1] 侯道荣[1] 徐荣[1] 夏彪[1] 德伟[1] 王心如[2] 

机构地区：[1]南京医科大学生化与分子生物学系,江苏南京210029 [2]南京医科大学毒理学系,江苏南京210029

出　　处：《现代生物医学进展》2010年第11期2057-2060,共4页Progress in Modern Biomedicine

基　　金：江苏省国际合作项目(BZ2007075)

摘　　要：目的:表达人卵泡刺激素受体(follicle-stimulating hormone receptor,FSHR)N端第18-34氨基酸片段。方法:将FSHRN端第18-34氨基酸片段克隆至pGEX4T-1中,构建成重组质粒pGEX4T-1-FSHRN(18-34位氨基酸)。将该重组质粒转化E.coliBL21后,IPTG诱导其表达,经亲和层析进行分离纯化,western blot鉴定。结果:克隆成功,并表达FSHRN端第18-34氨基酸片段,所表达的融合蛋白中60%为可溶性蛋白。结论:成功克隆、表达、纯化了人FSHRN端第18-34氨基酸片段,为后续动物免疫试验提供抗原。Objective：To produce the N-terminal 18-34 aa fragment of follicle-stimulating hormone receptor.Methods：The N-terminal 18-34 aa fragment of follicle-stimulating hormone receptor was cloned and translated into pGEX4T-1 vector.The expression plasmid was translated into E.coli strain BL-21.The GST fusion protein was induced by IPTG and purified to homogeneity by affinity chro-matography;Then protein was identified by western blot.Results：The recombinant GST-FSHR N fusion protein was expressed and purified successfully, of which 60% was soluble.Conclusion：The expression and purification of N-terminal 18-34 aa fragment of follicle-stimulating hormone receptor would provide antigen for the further animal experiment.

关 键 词：FSHR 表达 纯化 

分 类 号：Q78[生物学—分子生物学]