钙激活性氯离子通道的电生理研究  

Research on the electrophysiological properties of the calcium-activated chloride ion channels currents

作　　者：曾闵[1] 庞玉生[1] 韩玉兰[1] 韦宇明 

机构地区：[1]广西医科大学第一附属医院儿科,广西南宁530021 [2]广西卫生干部管理学院,广西南宁530021

出　　处：《现代生物医学进展》2010年第11期2064-2066,共3页Progress in Modern Biomedicine

基　　金：广西自然科学基金(桂科自0991133);广西研究生教育创新计划资助项目(2009105981002M219)

摘　　要：目的:研究大鼠肺动脉平滑肌细胞上钙激活性氯离子通道的电流、电压电流关系等电生理特性。方法:采用急性酶分离法(胶原酶Ⅰ型和木瓜蛋白酶)分离出单个肺动脉平滑肌细胞,应用膜片钳技术,测定各组大鼠肺动脉平滑肌细胞上钙激活性氯离子通道电流和电压电流。结果:急性酶分离法能成功分离出适用于膜片钳记录的单个大鼠肺动脉平滑肌细胞,并测到稳定的钙激活性氯离子通道电流,该电流表现出时间、电压及钙离子依赖性,并呈外向整流特征。结论:大鼠肺动脉平滑肌细胞的钙激活氯离子通道具有时间依赖性、钙离子依赖性和电压依赖性,并具有外向整流特征。Objective：To investigate the electrophysiological properties of the calcium-activated chloride ion channels currents in PASMCs.Methods：PASMCs were obtained by the acute emzyme separation method （collagenase I and plus papain） and the electro-physiologicalpropertiesofthe calcium-activated chloride ion channels was detected bythe conventional whole-cell patch technique.Results：The isolated PASMCs were suitable for patch clamp recording.Stability calcium-activated chloride ion channel currents were recorded.This kind of currents showed time-dependent, voltage-dependent and calcium-dependent, and which had outwardly rectifying characteristics.Conclusion：The current of calcium-activated chloride channels in rat pulmonary arterial smooth muscle cells are time-dependent, calcium-dependent and voltage-dependent, and which had outwardly rectifying characteristics.

关 键 词：肺动脉平滑肌细胞 急性酶分离 钙激活性氯离子通道 膜片钳 

分 类 号：Q95-3[生物学—动物学] Q46