粗山羊草居群间遗传分化及多样性的SSR分析  

Genetic diversity and differentiation in different Aegilops tauschii populations revealed by SSR

作　　者：苏亚蕊[1] 张大乐[1] 徐守明[1] 高安礼[1] 黄世全[1] 李锁平[1] 

机构地区：[1]河南大学生命科学学院,农业生物技术研究所,河南开封475004

出　　处：《生态学报》2010年第4期969-975,共7页Acta Ecologica Sinica

基　　金：国家自然科学基金资助项目(30971774);河南省高校杰出人才创新工程资助项目(HAIPURT,2007KYCX009)

摘　　要：选用分布在粗山羊草14条染色体上的32对SSR引物,对来自中国河南、陕西、新疆和中东地区共147份粗山羊草材料进行遗传分化及多样性分析,结果表明在26个多态性位点中,等位基因数平均为4.15,Ne i基因多样性指数(He)平均为0.243,多态性信息含量指数(PIC)平均为0.226;居群间遗传变异差异明显,中东粗山羊草居群具有丰富的遗传变异(He=0.607,PIC=0.551),而来自陕西和河南的粗山羊草资源遗传多样性较低(He=0.055,PIC=0.047)和(He=0.024,PIC=0.021)。AMOVA分子变异分析显示,居群间遗传变异占总变异的52%,达到显著水平;河南粗山羊草和陕西粗山羊草间发生了一定的遗传分化(Fst=0.210),为研究中国粗山羊草资源的起源与分化问题提供了有用的信息与证据。The genetic diversity and differentiation of 147 Aegilops tauschii accessions from Henan,Shannxi,Xinjiang in China and the Middle East,were investigated by SSR assay with 32 pairs of primers homogeneously distributed on 14 chromosomes of Ae.tauschii.The results demonstrated that 26 loci were polymorphic,with an average of 4.15 alleles on each locus.Nei＇s genetic diversity index（He） and polymorphism information content（PIC） value were 0.243 and 0.226,respectively.Genetic variations among different populations varied greatly.The maximal diversity（values were found in Middle East population（He =0.607,PIC =0.55 1）,which was much higher than those in Shannxi（He =0.055,PIC =0.047） and Henan（He =0.024,PIC =0.02 1） populations.Analysis of molecular variance（AMOVA） showed that the genetic differentiation among the geographical populations was significant（P〈0.05） and accounted for about 52% of the total genetic variation.Meanwhile,the genetic differentiation was obvious between the populations of Henan and Shannxi（Fst =0.2 10）.These results would offer useful information and evidence for further understanding the origin and evolution of the Ae.tauschii from China.

关 键 词：粗山羊草 SSR标记 遗传分化 遗传多样性 

分 类 号：S512.1[农业科学—作物学]