利用F_v/F_m检测锥状施克里普藻N和P限制的局限  

The limitation of maximal quantum yield of photosystem Ⅱ for the detection of nitrogen limitation and phosphorus limitation of Scrippsiella trochoidea

作　　者：齐红菊[1] 王江涛[1] 王昭玉[1] 

机构地区：[1]中国海洋大学海洋化学理论与工程技术教育部重点实验室,青岛266100

出　　处：《生态学报》2010年第8期2049-2055,共7页Acta Ecologica Sinica

基　　金：国际科技合作重点项目计划资助项目(2004DFA03600);国家高科技研究发展计划资助项目(2006AA09Z176)

摘　　要：通过测定缺N和缺P的锥状施克里普藻(Scrippsiella trochoidea)在分别添加充足的NO3-N、PO4-P后,光系统Ⅱ(PSⅡ)最大光化学量子产量(Fv/Fm)的变化,来检验Fv/Fm能否用来检测锥状施克里普藻N、P限制的情况。实验表明,锥状施克里普藻分别在不加N、P元素的f/2-Si培养基培养过程中,缺N组叶绿素a(Chla)浓度先升高,在原培养液N耗尽后快速下降;缺P组Chla开始时升高,在原培养液P耗尽后缓慢下降。两组藻液Fv/Fm比值保持相对稳定,短时间内未见明显降低。当分别向藻液中重新添加充足的N和P后,缺N组藻液添加N后Chla浓度48h内明显上升,缺P组藻液添加P后Chla浓度48h内没有明显变化,两种培养液中Fv/Fm均未见明显升高。结果证明,利用Fv/Fm不能够快速检测锥状施克里普藻N、P限制的情况,该法在检测浮游植物营养盐胁迫时具有局限性。The effects of different nutrient supply on the maximal quantum yield of photosystem Ⅱ（Fv/Fm） to Scrippsiella trochoidea in nitrate-starved and phosphorus-starved cultures were monitored in order to find weather it could be used to determine N and P limitation of S.trochoidea.The results showed that Chl a contents of N-starvation culture increased at first and then declined significantly after nitrate was exhausted,while Chl a contents of P-starvation culture increased initially and then declined slowly after phosphate was exhausted.Fv /Fm remained stable throughout the N-and P-starved incubation.When sufficient nitrogen and phosphorus were resupplied to N-and P-starvation cultures of S.trochoidea respectively,Chl a contents increased rapidly in 48 hours in N-starvation culture while it showed no obvious change in P-starvation culture.There was no measurable increase of Fv/Fm to nutrient addition in both of the two cultures.Our study demonstrates that Fv/Fm cannot be used for the detection of nitrogen limitation and phosphorus limitation of S.trochoidea.This method has limitations on the detection of nutrient stress for phytoplankton.

关 键 词：锥状施克里普藻 N限制 P限制 FV/FM 

分 类 号：Q945[生物学—植物学]