絮凝活性产紫青霉EL-02的分离鉴定及其絮凝活性初探  

Flocculating activity of Penicillium purpurogenum EL-02 and its flocculating activity

作　　者：张雨山[1] 王静[1] 郝建安[1] 张秀芝[1] 成玉[1] 

机构地区：[1]国家海洋局天津海水淡化与综合利用研究所,天津300192

出　　处：《微生物学报》2010年第7期917-922,共6页Acta Microbiologica Sinica

基　　金："十一五"国家科技支撑计划项目(2006BAB03A08)~~

摘　　要：【目的】分离鉴定有絮凝活性真菌,同时对其絮凝活性进行初步研究。【方法】采用梯度稀释、平板划线、18SrDNA检测等方法分离鉴定絮凝活性菌株。通过高速离心、超声破碎、乙醇沉淀、定性试验等方法确定絮凝活性物质性质。【结果】从渤海湾海岸土壤样品中分离筛选出一株有较高絮凝活性的真菌,经鉴定为产紫青霉(Penicillium purpurogenum),命名为产紫青霉EL-02(P.purpurogenum EL-02)。超声破碎试验证实其絮凝活性主要存在于发酵上清液。根据絮凝活性曲线,确定4d为积累絮凝活性产物的最佳发酵时间。乙醇沉淀法获得该菌株絮凝活性物质。经鉴定该菌株所产絮凝活性物质为糖类,且其活性在pH2-11,温度-70℃-100℃范围内保持稳定。【结论】分离筛选到一株有絮凝活性的产紫青霉EL-02,经鉴定其产生糖类絮凝活性物质。[Objective]To isolate,identify and characterize a fungus with flocculating activity.[Methods]We used gradient dilution,plaque distribution and 18S rDNA analysis to isolate and identify a fungus with flocculating activity.We used high-speed centrifugation,ultrasonication and qualitative test to determine the nature of flocculating active substances.[Results ] We isolated a flocculating active fungi and identified it as Penicillium purpurogenum.Ultrasonication test confirmed that the flocculating activity was primarily in the fermentation supernatant.We explored the flocculating activity curve and found that 4 days was the optimum fermentation time for accumulating flocculating active substances.The strain flocculating activity remains unchanged when pH varied from 2 to 11 and temperature varied from-70 ℃ to 100 ℃.We finally identified the flocculating active substances as saccharides.[Conclusion]We isolated a flocculating active strain P.purpurogenum EL-02,and identified its flocculating active products as saccharides.

关 键 词：絮凝活性 真菌分离鉴定 活性物质 产紫青霉 

分 类 号：X703.5[环境科学与工程—环境工程]