新疆羊粪便戊型肝炎病毒RNA的检测与序列分析  

Detection and sequences analysis of sheep hepatitis E virus RNA in Xinjiang Autonomous Region

作　　者：王永霞[1] 马勋[1] 

机构地区：[1]石河子大学动物科技学院,石河子832003

出　　处：《微生物学报》2010年第7期937-941,共5页Acta Microbiologica Sinica

基　　金：国家自然科学基金(30760012);新疆生产建设兵团科技局博士基金(2007JC15);国际科技合作项目(2006DFA33740)~~

摘　　要：【目的】为了了解新疆地区羊群中是否存在戊型肝炎的感染,我们从戊型肝炎病毒(Hepatitis E virus,HEV)IgG检测阳性的新疆某羊场采集54份1-3岁的羊粪便,【方法】利用逆转录套式聚合酶链方法(RT-nPCR),检测HEVRNA,其中6份为阳性,阳性率11.11%。【结果】将PCR扩增产物克隆,测序并进行序列分析,结果表明,6株羊源HEV检测株在HEV ORF2 189bp 99.38%-100%,为同一基因型;与HEVⅠ、Ⅱ、Ⅲ和Ⅳ型的同源性分别为78.67%-85.33%、81.33%-82.67%、78.67%-84.00%和84.67%-95.36%,与Ⅳ型最高同源性达94.04%-95.36%。以该189bp片段绘制进化树,发现与新疆牛源分离株、中国大陆猪源分离株(FJ610232)和中国大陆人源分离株(AJ272108)在同一分枝上,同属基因Ⅳ型;【结论】提示新疆羊群中可能存在HEV感染,并且羊可能是人类HEV传染源中除猪之外的新宿主。[Obiective]To understand whether hepatitis E virus （HEV） was infectious in sheep in Xinjiang.[Methods] We used reverse transcription-nested polymerase chain reaction（RT-nPCR） to detect HEVRNA in feces.The feces were collected from sheep with positive anti-HEV antibodies in a sheep farm in Xinjiang Autonomous Region.[Results]Six of 54 （11.11%） sheep were positive for HEV RNA.PCR amplification products were cloned,sequenced and analyzed.The homology among HEV of the 6 sheep HEV ORF2 189bp nucleotide amplification sequences was 99.38%-100%.They should belong to the same genotype.They were respectively compared with HEV genotype Ⅰ,Ⅱ,Ⅲ and Ⅳ corresponding 189bp nucleotide sequences.The average homology was 78.67%-85.33%,81.33%-82.67%,78.67%-84.00% and 84.67%-95.36%.The maximum homology between 6 nucleotide amplification sequences and one sequence of genotype Ⅳ was 94.04%-95.36%.Based on sequence of the nucleic acid fragments,a phylogenetic tree was constructed.Six sheep HEV ORF2 189bp nucleotide amplification sequences in this study and bovine HEV,swine HEV and human HEV locate the same evolutionary vine and belong to genotype Ⅳ.[Conclusion]The finding suggested that infection of HEV probably exists in the sheep group in Xinjiang Autonomous Region and the sheep may be a new animal host except swine in origins of HEV infection.

关 键 词：戊型肝炎病毒 基因型 RT-nPCR 羊 

分 类 号：S852.65[农业科学—基础兽医学]