聚乙二醇作为乙型肝炎病毒DNA疫苗佐剂的体外试验  

Effect of polyethylene glycol as adjuvant on hepatitis B virus DNA vaccine in vitro

作　　者：王军朋[1,2] 芦丽淦[1,2] 宋红利[3] 杨永杰[1,2] 马远方[1,2] 

机构地区：[1]河南大学医学院细胞与分子免疫学重点实验室,开封475001 [2]河南大学免疫研究所,开封475001 [3]河南大学体育学院,开封475001

出　　处：《微生物学报》2010年第7期949-954,共6页Acta Microbiologica Sinica

基　　金：河南大学科研启动基金~~

摘　　要：【目的】研究聚乙二醇作为佐剂是否能够增强HBVDNA疫苗体液和细胞免疫效果。【方法】我们将乙型肝炎病毒(HBV)DNA疫苗(pVAX-S2)单独或PEG/pVAX-S2免疫C57BL/6J小鼠,在最后一次免疫14d检测抗-HBsIgG、T淋巴细胞增殖、细胞因子表达及体内细胞毒T淋巴细胞杀伤作用(CTL)等免疫学指标。【结果】结果表明,与仅免疫pVAX-S2比,PEG/pVAX-S2能够增加免疫小鼠抗-HBsIgG水平;混合免疫组的T淋巴细胞体外经乙肝表面抗原(HBsAg)刺激后,T淋巴细胞增殖数量显著高于对照组;另外,混合免疫组CD4+T淋巴细胞中IL-4和IFN-γ以及CD8+T淋巴细胞中IFN-γ的表达显著高于对照组。更重要的是,混和免疫组HBsAg特异性CTL显著高于对照组。【结论】总之,我们首次证明PEG作为佐剂不仅能够增强体液免疫反应和细胞免疫反应,而且能够显著增强体内CTL的杀伤活性,为HBV DNA的进一步研究奠定了基础。[Objective]To explore whether polyethylene glycol （PEG） as adjuvant could enhance the humoral and cellular immune responses on hepatitis B virus DNA vaccine.[Methods]C57BL /6J mice were immunized with PEG and pVAXS2 or alone pVAX-S2.After these mice were finally immunized for 14 days,the anti-HBs IgG,T cell proliferation,the expression of cytokines and CTL in vivo were detected.[Results]Compared to mice immunized with alone pVAX-S2,PEG as adjuvant could increase the production of anti-HBs IgG and HBsAg specific T cell proliferation.In addition,the expression of IL-4,IFN-γ in CD4 ＋ T cells and IFN-γ in CD8 ＋ T cells was higher than control groups.The PEG /pVAX-S2 groups could elicit significantly in vivo HBsAg specific CTL responses.[Conclusions]PEG as adjuvant could enhance humoral and cellular immune responses,as well as in vivo CTL activity.

关 键 词：聚乙二醇 乙型肝炎病毒(HBV)DNA疫苗 体液免疫反应 细胞免疫反应 

分 类 号：R392[医药卫生—免疫学]