An in-depth analysis of proteomics expression profiling in rat glomeruli utilizing LC-MS  

作　　者：HONG Quan XUE Peng LU Yang CHEN XiangMei QI Ka WU Di 

机构地区：[1]Kidney Department & Institute of Nephrology, Division of Clinical Internal Medicine, Chinese PLA General Hospital, Beijing 100853, China [2]Laboratory of Proteomics, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China

出　　处：《Chinese Science Bulletin》2010年第20期2142-2151,共10页

基　　金：supported by the National Natural Science Foundation of China(30670983)

摘　　要：Glomeruli are an essential functional element of renal filtration.The majority of renal diseases caused by glomerular sclerosis or fibrosis may result in renal dysfunction.A fomulate protein profile,a comprehensive analysis of glomeruli of normal rats was conducted in this study via protein spectrum.Functional annotation and classification of these proteins were performed and it was found that 26 had the same glomerule(endothelial cells,podocytes and mesangial cells)markers with proteins.Glomemli are an essential functional element of renal filtration. The majority of renal diseases caused by glomerular sclerosis or fibrosis may result in renal dysfunction. A fomulate protein profile, a comprehensive analysis of glomeruli of normal rats was conducted in this study via protein spectrum. Functional annotation and classification of these proteins were performed and it was found that 26 had the same glomemle （endothelial cells, podocytes and mesangial cells） markers with proteins.

关 键 词：蛋白质组学 肾小球 大鼠 MS法 LC 作者 内皮细胞 肾功能不全 

分 类 号：Q51[生物学—生物化学] S856.59[农业科学—临床兽医学]