邻苯二甲醛衍生荧光法测定蔬菜中残留克百威  

Determination of Carbofuran Residues in Vegetable by Fluorospectrophotometry with o-Phthalaldehyde Derivatization

作　　者：代永佳[1] 陈冠华[1] 石杰[1] 孙娟[1] 董敏[1] 

机构地区：[1]江苏大学食品与生物工程学院,江苏镇江212013

出　　处：《广西师范大学学报（自然科学版）》2010年第2期61-64,共4页Journal of Guangxi Normal University:Natural Science Edition

基　　金：国家自然科学基金资助项目(30970309);江苏大学科研基金资助项目(08JDG001)

摘　　要：为了测定蔬菜中克百威残留，建立了测定残留克百威的荧光分析新方法。样品用乙酸乙酯提取，经活性炭和克百威固相萃取柱净化后，在pH8．0的沸水中水解25min，在pH11．25的碳酸盐缓冲液中与邻苯二甲醛衍生，邻苯二甲醛与克百威的质量比不小于6．5：1。在此条件下，克百威检测的线性动态范围是0．09～1．2mg／L，检出限为25ug／kg，加标回收率为82．18％-94．3%。方法可用于蔬菜样品中克百威残留的测定。The new fluroscent assay has been developed for determining carbofuran residue in vegetable. The sample is extracted with acetic ether,cleaned up with activated carbon and carbofuran solid phase extracting column,hydrolyzed for 25 min in boiled water of pH8. 0,derivatized with o-phthalaldehyde in carbonate buffer of pH11. 25 and the mass ratio of o-phthaladehyde and carbofuran is more than 6.5 ： 1. Under these conditions,the linear dynamic range of carbofuran is 0. 09-1.2 mg/L,detection limit is 25 ug/kg and the recoveries of spiked sample are between 82.18%-94. 3%. The assay can be applied for determination of carbofuran in vegetables.

关 键 词：残留克百威 邻苯二甲醛 水解 衍生 荧光分光光度法 

分 类 号：O657.3[理学—分析化学]