两株微藻的分离鉴定及Fe^3＋对其生长和脂质积累的影响  

Isolation and Identification of Two Strains of Microalgae and the Effect of Fe^(3+) on Their Growth and Lipid Accumulation

作　　者：夏金兰[1] 李丽[1] 万民熙[1] 刘鹏[1] 王润民[1] 黄斌[1] 邱冠周[1] 

机构地区：[1]中南大学资源加工与生物工程学院j生物冶金教育部重点实验室,湖南长沙410083

出　　处：《武汉大学学报（理学版）》2010年第3期325-330,共6页Journal of Wuhan University:Natural Science Edition

基　　金：国家自然科学基金资助项目(50321402)

摘　　要：自青岛汇泉湾海水样品中分离培养两株微藻HQW01和HQW02,经形态和系统发育分析,两株微藻分别鉴定为三角褐指藻(Phaeodactylum tricornutum)和球等鞭金藻(Isocrydid galbana).采用吸光度和尼罗红荧光染色法研究了Fe3+对这两株微藻生长和脂质积累的影响.结果表明,三角褐指藻在Fe3+浓度为1×10-4mol/L培养时,生长速度最快且脂质含量最高,达到47.3%,是不加Fe3+培养时的4.2倍;Fe3+浓度为1×10-5mol/L时,球等鞭金藻具有最大生长速率,同时脂质含量提高至41.9%,是不加Fe3+培养时的2.8倍.这两种高脂质含量的微藻有望作为生物柴油的生产原料.The strains HQW01 and HQW02 were isolated from Hui-quan Bay,Qingdao City.They were identified as Phaeodactylum tricornutum and Isochrysis galbana respectively based on the morphology characteristics and systematics analysis.The effect of different concentrations of Fe3＋ on their growth and lipid accumulation were detected by absorbance and fluorescence intensity respectively.The results showed that the strain HQW01 had the highest growth rate and lipid content at 1×10-4 mol/L Fe3＋ concentration and the lipid content was 47.3% which was 4.2 times compared with the controlled.For strain HQW02,the optimal concentration of Fe3＋ was 1×10-5 mol/L and the lipid content increased to 41.9% which was 2.8 times compared with the controlled.These two microalgal strains which had higher lipid content could be the materials to produce biodiesel.

关 键 词：微藻 18SrRNA FE3+ 脂质积累 尼罗红荧光染色法 

分 类 号：TK6[动力工程及工程热物理—生物能] Q949.2[生物学—植物学]