细胞融合技术及其在构建水处理工程菌中的应用  

On the cell-fusion technique and Its applications to the construction of sewage treatment engineering strains

作　　者：任柏林[1] 谢水波[2] 唐振平[2] 刘迎九[1] 

机构地区：[1]南华大学污染控制与资源化技术湖南省高校重点实验室,湖南衡阳421001 [2]南华大学铀矿冶生物技术国防重点学科实验室,湖南衡阳421001

出　　处：《安全与环境学报》2010年第3期20-24,共5页Journal of Safety and Environment

基　　金：国家自然科学基金项目(10775065);湖南省高校创新平台开放基金项目(09K076)

摘　　要：为探讨细胞融合技术在构建水处理工程菌中的应用,从细胞融合的原理与发展史、常用细胞融合方法及细胞融合的最新进展等方面对细胞融合技术进行了介绍。比较分析了常用的病毒法、化学试剂法、电融合、激光融合、灭活原生质体融合等方法的原理、优缺点,介绍了最新发展起来的基于微流控芯片、高通量细胞融合芯片以及离子束的细胞融合技术。通过对原生质体融合技术在含氯、聚乙烯醇(PVA)、重金属等其他废水的处理研究实例探讨,展示了细胞融合技术在构建水处理工程菌中的应用前景,并对融合菌应用中存在的问题进行了分析,为利用细胞融合技术构建水处理工程菌提出了发展方向。This paper is focused on introducing our research results of the characteristic features of the so-called cell fusion technque and the ways of its application to constructing engineering strains for sewage treatment ,Since the cell fusion technique is likely to be the one unfamiliar to our readers ,we would like to offer a brief presentation on it from its working priciple,developmental history,some popular fusion methods as well as its latest advancements.As we know,it enjoys a long history,which is acknowledged as a starting point：As a matter of fact,Okada found in 1958 that Sendai virus could be brought into ascites to make the cancer cell fuse.Since then,quite a number of fusion methods have been introduced,such as virus cell fusion,chemical-fusion ,electro-fusin ,laser-fusion,inactivated protoplasts fusion,etc.Next,we would like to make a comparison between its principle,its adyantages and disadvantages. The latest developments of the technique may involve microfluidic chip-based,high-throughout cel fusion chips ,as well as the ion beam cell fusion ,all of which help to result in the highly-efficient modern electronic and chip technique,though not yet widely used.The branches of the technique can be said to stand for its working principles in different areas,with some of them used to treat sewage containing chlorine,polyvinyl alcohol（PVA）,heavy metals and other industrial waters by protoplast fusion .THe results of the wide application of the technique,significant and fruitful,suggesting a great potential in further application .And,finally,we have also analyzed problems with its application ,such as security problem and so on .However,it is also necessary to point out its development directions for the cell fusion technique in constructing engineering strains of water treatment.

关 键 词：细胞生物学 细胞融合 水处理 工程菌 

分 类 号：TS201[轻工技术与工程—食品科学]