泥浆生物反应器中高相对分子质量多环芳烃生物降解及影响因素研究  

On the characteristics and affecting factors of the biodegradation of the high molecular weight PAHs as a soil-slurry reactor

作　　者：陈海英[1] 丁爱中[1] 豆俊峰[1] 范福强[1] 杜勇超[1] 李晓斌[1] 

机构地区：[1]北京师范大学水科学研究院,地下水污染控制与修复教育部工程研究中心,北京100875

出　　处：《安全与环境学报》2010年第3期53-57,共5页Journal of Safety and Environment

基　　金：北京市科技计划项目(D08040900360804)

摘　　要：泥浆生物反应器技术是有效修复多环芳烃(PAHs)污染土壤的异位处理方法。利用正交试验(L_9(3~4)),研究在泥浆生物反应器内接种混合菌对土壤中高相对分子质量PAHs(苯并[b]荧蒽、苯并[k]荧蒽和苯并[a]芘)的生物降解及影响降解效果的因素,得到最优的降解工艺条件。12 d后苯并[b]荧蒽、苯并[k]荧蒽和苯并[a]芘的最高降解率分别为54.72%、54.01%和63.13%。在试验范围内,影响土壤中PAHs生物降解的显著因素为通气量、水土比和pH值,盐度无显著影响。PAHs生物降解的最优工艺条件是水土比为2:1,通气量为200L.h^(-1),盐度为1.5%,pH值为7.0。This paper aims to study the biodegradability of a mixture of high molecular weight PAHs, namely benzo[ b]-fluoranthene, benzo[ k]-fluoranthene, benazo[ a]-pyrene, so as to determine the factors influencing their biodegradation through their optimization. As is known, bio-slurry reactor technology is one of the highly effective and eco-friendly ex-situ treatments for remediating PAH-contaminated soil. However, the biodegradation of PAHs can be affected by their physical, chemical and biological factors, such as pollution concen- tration, nutrition, microbial activities, bioavailability, dissolved oxygen and so on. Therefore, it is of great significance to isolate the affective bacteria from their ability to degrade PAHs and optimize the environmental parameters affecting the biodegradation. In proceeding with our study, we have successfully screened off and enriched the mixed bacteria from PAHs contaminated soil from Beijing Coke Plant for our case study. Furthermore, we have set up orthogonal experimental design （L9 （3^4 ）） to investigate the degradation process in the bioslurry reactor with inoculation of the mixed bacteria and the effects of multi-factors on biodegradation, including the aeration rate, soil water ratio, pH value and salinity. Analysis of variance （ANOVA） was also adopted to make secure the experimental data so as to prove the significance of each factor and determine the optimal levels of the chosen factors for the biodegradation. The results of our investigation indicate that the above said PAHs could be degraded effectively in bio-slurry reactors with the inoculation of mixed bacteria. The highest degradation efficiency of the PAHs was found to be 54.72%, 54.01% and 63.13% respectively 12 days later. Among the factors to be taken into account, three of which, that is, aeration, rate water soil ratio, and pH, serve as significant ones （p 〈 0.05）, whereas salinity can be thought of as insignificant （ p 〉 0.05 ）. Taking both F values and p values into account, aeration r

关 键 词：环境工程学 泥浆生物反应器 高相对分子质量PAHs 生物降解 正交试验设计 

分 类 号：X53[环境科学与工程—环境工程]